Wisconsin Alumni Research Foundation

Scarab Genomics, LLC

Jeremy D Glasner

Frederick R Blattner

Gyorgy Posfai

Guy Plunkett

Christopher D Herring

Trevor Twose

Trevor Martin Twose

Guy D Plunkett

Twose Trevor

The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Bacteria with reduced genome

The present invention discloses that a bacterium having a genome that is genetically engineered to be at least 10% smaller than the genome of its native parent strain has better transformation competence. Specificstrains, having significantly reduced genome sizes, are disclosed which are highly transformation competent. A medium and methodology is taught which enables transformation efficiencies to be increased further.

Competent bacteria

Growing community of inventors

Madison, WI, United States of America

Jeremy D Glasner