Foss Analytical A/s

Jeppe Sandvik Clausen

Per Waaben Hansen

A method for determining an optical pathlength through a cuvette of a spectrophotometric apparatus includes obtaining a first single beam spectrum of a liquid zero-material at least in a first energy region in which the liquid zero-material absorbs at least a portion of incident optical radiation; obtaining a second single beam spectrum of a second liquid at least in the first energy region, the second liquid having a composition excluding the liquid zero-material and having no absorption of incident optical radiation in the first energy region; determining a dual beam spectrum of the liquid zero-material relative to the second liquid at least in the first energy region from the first and second single beam spectra; and calculating an optical pathlength through the cuvette based on processing spectral information obtained from the first energy region of the determined dual beam spectrum.

Method of determining an optical pathlength through a cuvette

A method of correcting for an amplitude change in a spectrometric instrument output includes: exposing a sample in a sample holder to electromagnetic radiation at a plurality of wavenumbers; detecting electromagnetic absorption intensities in the sample at the plurality of wavenumbers; providing to a computer device the detected electromagnetic absorption intensities indexed against wavenumber as spectral data; applying in the computer device a mathematical transform (Icorr) to the spectral data to correct for an amplitude change in the spectrometric instrument's output and calculated by determining a difference (Δ(SBZ)/) between first derivatives of a logarithmic transformation of spectral data (SB) from the zero material sample at two different wavenumber ranges (log(SB(x))and log(SB(x))); and calculating the mathematical transform (Icorr) as a function inversely dependent on the determined difference (Δ(SBZ)/).

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Hilleroed, Denmark

Jeppe Sandvik Clausen