Korea Research Institute of Bioscience and Biotechnology

Korea Research Institute of Standards and Science

Jeong Hee Moon

Tae Geol Lee

Bong Hyun Chung

Tae Sung Yoon

Jeong Hoon Kim

Dae Won Moon

Byoung Chul Park

Dae Gwin Jeong

Ju Hwang Kim

Myung Kyu Lee

Seong Eon Ryu

Yong-Hyeon Yim

Sohee Yoon

Ji-Seon Oh

Seok-II Hong

Sung Woo Heo

Sung Goo Park

Hyoung Jun Lee

Ga Bi Lee

Young Joon Hong

A mass spectrometry device includes a sample seating part including an ultrasonic vibrator having a through hole through which liquid particles formed by the ultrasonic vibrator from an adsorbent material including a sample and a solvent are discharged, the adsorbent material being seated on the ultrasonic vibrator; a reaction part in which plasma or an ionization medium generated by plasma come into contact with the liquid particles discharged from the through hole to form an ionized material; an introduction part discharging and introducing the ionized material to a detection part; and the detection part analyzing the ionized material discharged from the introduction part. The mass spectrometry device and the mass spectrometry method can detect the components of various samples by converting a sample into liquid particles using ultrasonic waves and applying plasma and can detect samples in various fields without regard to locations.

Ionization mass spectrometry method and mass spectrometry device using same

Provided is a methionyl tRNA synthase (MRS) for the biosynthesis of a photomethionine-labeled protein and a method for preparing a photoactivatable protein G variant using same and, more particularly, to an MRS variant in which alanine at the position of 12is substituted with glycine, leucine at the position of 13by serine, tyrosine at the position of 260by phenylalanine, isoleucine at the position of 297by valine, and histidine at the position of 301by leucine from the N-terminal of the amino acid sequence of a wild-typemethionyl tRNA synthase. The MRS variant effectively confirms the biosynthesis of a photomethionine (pM)-labeled target protein and thus can be utilized for the biosynthesis of a pM-labeled target protein. In addition, a pM-introduced protein G variant, in which a plasmid encoding the MRS variant (MRS5m) and a PG-C3 plasmid, in which, in the third immunoglobulin G binding region of protein G, positions of 32, 35, and 40are substituted with a methionine (Met) residue and a position of 37by an arginine (Arg) residue, are introduced intoand then refined, has a specific covalent bond with an antibody when subject to UV irradiation, and thus the pM-introduced protein G variant using the MRS variant can be utilized for producing a highly sensitive biochip, biosensor, or cell-capturing chip.

Methionyl tRNA synthetase for biosynthesis of photomethionine-labeled protein and method for preparing photoactive protein G variant using same

The present invention aims to provide a time-of-flight based mass microscope system for an ultra-high speed multi-mode mass analysis, for using a laser beam or an ion beam simultaneously to enable both a low molecular weight analysis such as for drugs/metabolome/lipids/peptides and a high molecular weight analysis such as for genes/proteins, without being limited by the molecular weight of the object being analyzed, and for significantly increasing the measuring speed by using a microscope method instead of a microprobe method.

Flight time based mass microscope system for ultra high-speed multi mode mass analysis

The present invention relates to a method for quantifying protein tyrosine phosphatase (referred as PTP hereinafter) in biosamples, precisely a diagnostic method for disease by quantifying PTP using mass spectrometry and profiling of comparative PTP levels. By quantifying PTP in biosamples and profiling thereof according to the method of the present invention, disease can be diagnosed and diverse disease conditions and health conditions can be confirmed via profiling.

Method for diagnosis of disease using quantitative monitoring of protein tyrosine phosphatase

Growing community of inventors

Daejeon, South Korea

Jeong Hee Moon