Novo Nordisk A/s

Novozymes A/s

Novozyme A/s

Jens Sigurd Okkels

Allan Svendsen

Marianne Thellersen

Shamkant Anant Patkar

Jesper Vind

Kim Borch

Torben Halkier

Claus Crone Fuglsang

Ib Groth Clausen

John C Royer

Steen Troels Jorgensen

Erik Gormsen

Titus Kretzschmar

Dorte Aaby Petersen

Tomoko Matsui

Shiro Fukuyama

Tadashi Yoneda

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Lipolytic enzymes

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

The invention provides variant lipases with improved washing performance, including good performance in washing with a detergent having a high content of anionic surfactant at low washing temperature at a short washing time. More particularly, the invention relates to variants of the wild-type lipase from Pseudomonas sp. strain SD 705, deposited as FERM BP-4772.

Lipase variants

The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.

Method of preparing a variant of a lipolytic enzyme

The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.

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Frederiksberg C, Denmark

Jens Sigurd Okkels