Biopetrolia Ab

Melt & Marble Ab

Firmenich Sa

Chrysea Limited

Novozymes A/s

Danmarks Tekniske Universitet

Total Raffinage Chimie

Elypta Ab

Jens Nielsen

Anastasia Krivoruchko

Florian David

Verena Siewers

Michel Schalk

Tao Yu

Laurent Daviet

Paulo Alexandre Goncalves Teixeira

Yongjin Zhou

Nicolaas A A Buijs

Shuobo Shi

Mingtao Huang

Yiming Zhang

Zongjie Dai

Henrik Pedersen

Carsten Mailand Hjort

Bo Jiang

Francesco Gatto

Christoph Knuf

Gionata Scalcinati

Yun Chen

Zhiwei Zhu

Torsten Ulrik Bak Regueira

Jiufu Qin

Leonie Wenning

Yong-Jin Zhou

The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

Fungal cell with improved protein production capacity

The present invention generally related to a modified microbial cell capable of producing high levels of spermidine and/spermidine derivatives. The genetically modified microbial cell comprises at least one modification to native spermidine biosynthetic pathway via putrescine together with genes involved in the S-adenosylmethionine biosyntheticpathway.

Microbial cells for spermidine production

A fungal cell is capable of producing high levels of fatty acids and fatty acid-derived products. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

Genetically modified fungal cells for overexpression of an acetyl-CoA carboxylase and a pyruvate carboxylase

The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.

Methods and products for production of wax esters

The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

Engineering of acetyl-CoA metabolism in yeast

The present invention relates to a method of screening for renal cell carcinoma in a subject, said method comprising determining the level and/or chemical composition of one or both of the glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) in a body fluid sample, wherein said sample has been obtained from said subject.

Cancer biomarkers

The present invention relates to a method for producing fatty acids, in particular shot/medium-chain fatty acids (S/MCFA), by expression in a host cell of a recombinant fatty acid synthase (FAS) naturally devoid of a thioesterase (TE) domain comprising a heterologous thioesterase (TE). The invention further relates to polynucleotides comprising such recombinant FAS, polypeptides encoded thereby, and vectors comprising such polynucleotides, as well as recombinant host cells comprising the polynucleotides, polypeptides, or vectors.

Method of producing fatty acids

The present invention generally relates to a genetically modified fungal cell capable of producing a very long, chain fatty acid (VLCFA) and/or a VLCFA derivative. The genetically modified fungal cell comprises at least one exogenous gene encoding a fatty acyl-CoA reductase, and at least one gene encoding an elongase, and/or at least one gene encoding a fatty acid synthase.

Fungal cells and methods for production of very long chain fatty acid derived products

The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine 'acetylated derivatives cycle', L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.

L-ornithine production in eukaryotic cells

The present invention relates to the development of genetically engineered yeasts that can produce hydrocarbons in a controllable and economic fashion. More specifically the invention relates to the production of liquid alkanes and alkenes that can be used for liquid transportation fuels, specialty chemicals, or feed stock for further chemical conversion.

Engineering of hydrocarbon metabolism in yeast

The present invention relates to the control of gene expression by a heterologous glucose-regulated promoter, to microorganisms in which gene expression is controlled by a heterologous glucose-regulated promoter and to methods using said microorganisms for the production of terpenes during glucose-limited fed-batch fermentation.

Modified microorganisms and use thereof for terpene production

Growing community of inventors

Gothenburg, Sweden

Jens Nielsen