Dublin City University

Jens Ducree

Luke P Lee

Macdara Glynn

Robert Burger

Ivan Dimov

David Kinahan

Charles Nwankire

Gregor Kijanka

Asif Riaz

Lourdes Basabe-Desmonts

Ram Prasad Gandhiraman

Stephen Michael Daniels

Sinead Kearney

Nikolay Dimov

Jennifer Gaughran

Louise Barrett

Elizaveta Vereshchagina

The present invention relates to fluidic devices, especially microfluidic devices, for aliquoting and pairwise combinatorial mixing of a first set of liquids with a second set of liquids. The device architecture is designed to move liquids in two separate phases, a first phase where the liquids are exposed to a first directional force field to move the liquids in a first direction, from a reservoir to aliquot chambers, and a second phase where the liquids are exposed to a second directional force field to move the liquids in a second direction, from the aliquot chambers to the mixing chambers. The first and second directional force fields that the device is exposed to may be achieved using a single directional force field (i.e. a rotor driven centrifugal force field) and by re-orienting the position of the device with respect to the centrifugal forces between the first and second phases of operation. The device architecture comprises reservoirs for each of the first fluids and reservoirs for each of the second fluids. Each reservoir is fluidically connected to aliquoting chambers, either arranged in parallel or in series, for providing aliquots of the fluid which may be metered. The conduits providing fluid communication between the reservoirs and aliquoting chambers are arranged in a first direction. A series of mixing chambers is also provided, and each mixing chamber is fluidically connected to one aliquot chamber for a first liquid and one aliquoting chamber for a second liquid. The conduits providing fluid communication between the aliquoting chambers and mixing chambers are arranged in a second direction.

Fluidic device for aliquoting and combinatorial mixing of liquids

Microfluidic devices that are configured to use centrifugal forces to bias particles into one or more capture regions based on their individual sizes are described.

Microfluidic device

A particle capture system that can be used in the context of a lab-on-a-chip platform for particle- and cell-based assays is described. The system comprises a capture chamber comprising a plurality of capture sites, the capture sites defining a capture area configured to receive individual particles travelling within the capture chamber. By rotating the chamber, the individual particles are biased towards the capture sites where they may be captured.

Centrifugal capture system

A sequential flow analysis tool comprising a microfluidic device having a fluid path defined within a substrate between an input and an output is described. The device includes a capture chamber provided within but offset from the fluid path, the capture chamber extending into the substrate in a direction substantially perpendicular to the fluid path such that operably particles provided within a fluid flowing within the fluid path will preferentially collect within the capture chamber.

Microfluidic multiplexed cellular and molecular analysis device and method

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Ashbourne, Ireland

Jens Ducree