Fluxome Sciences A/s

University of California

Evolva Sa

Novo Nordisk A/s

Firmenich Sa

Evola Sa

Forster, Jochen

Nielsen, Jens B.

Jens Bredal Nielsen

Jochen Förster

Michael Katz

Hans Peter Smits

Bernhard O Palsson

Imandokht Famili

Pengcheng Patrick Fu

Michel Schalk

Torben Lauesgaard Nissen

Michi Egel-Mitani

Anthony Clark

Morten C Kielland-Brandt

Jure Piskur

Nina Katarina Gunnarsson

Aradhana Srivastava

Birgitte Regenberg

Christoffer Bro

Bernard O Palsson

Kasper Møller

Mohammad Ali Asadollahi

Jérôme Maury

Pengcheng Fu

Jochen Forster

A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including, or

Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof

The invention provides an in silico model for determining aphysiological function. The model includes a data structure relating a plurality ofreactants to a plurality ofreactions, a constraint set for the plurality ofreactions, and commands for determining a distribution of flux through the reactions that is predictive of aphysiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silicomodel and methods for determining aphysiological function using a model of the invention.

[object Object]

The present invention relates to a microorganism capable of producing a terpene of choice. The microorganism expresses a heterologous pathway for the formation of isoprene units and, preferably, a heterologous terpene synthase. In this way, high amounts of terpene can be isolated from the medium of the microorganism.

Method for producing terpenes and MEP-transformed microorganisms therefore

The present invention relates to the construction and engineering of cells, more particularly microorganisms for producing PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of an oxygen requiring pathway. The invention especially involves improvement of the PUFA content in the host organism through fermentation optimization, e.g. decreasing the temperature and/or designing an optimal medium, or through improving the flux towards fatty acids by metabolic engineering, e.g. through over-expression of fatty acid synthases, over-expression of other enzymes involved in biosynthesis of the precursors for PUFAs, or codon optimization of the heterologous genes, or expression of heterologous enzymes involved in the biosynthesis of the precursor for PUFAs.

A recombinant prototrophic micro-organism such as a yeast is provided which over-expresses the activity of an enzyme catalysing the conversion of glucose-1 phosphate to glucose-6 phosphate in the galactose uptake and metabolism pathway. Suitably the increased enzyme activity is due to over-expression of the gene PGM2. This produces increased uptake of galactose and increased ethanol production when the organism is grown on a galactose containing medium.

Metabolically engineered micro-organisms having improved galactose uptake

A recombinant microbial cell comprises at least one increased expressible enzyme activity controlling anabolic metabolism of ammonia as a nutrient source, the increased enzyme activity being an NADH-dependent activity catalysing the reaction

Metabolically engineered microbial cell with an altered metabolite production

The invention describes industrial fermentation of a Saccharomyces yeast species for production of a heterologous product encoded by a plasmid or DNA contained in said Saccharomyces yeast species which method utilizes the substrate more efficiently and without fermentative metabolism resulting in formation of ethanol and other unwanted primary products of fermentative activity whereby high yields of the heterologous product are obtained. The Saccharomyces yeast species is preferably a Crabtree negative Saccharomyces species in particular

Method for the production of heterologous polypeptides in transformed yeast cells

Growing community of inventors

Charlottenlund, Denmark

Jens Bredal Nielsen