Other

Q-linea Ab

Strömberg, Mattias

Nilsson, Mats

Svedlindh, Peter

Göransson, Jenny

Strømme, Maria

Gunnarsson, Klas

Jenny Göransson

Mats Gullberg

Mats Nilsson

Peter Svedlindh

Klas Gunnarsson

Mattias Strömberg

Maria Strçmme

Camilla Russel

Maria Strømme

Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located' of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs&#x2032; of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site&#x2032; of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.

Padlock probe detection method

A novel detection or quantifying method for biological entities or molecules such as, but not limited to, DNA, microorganisms and pathogens, proteins and antibodies, that by themselves are target molecules or from which target molecules are extracted, comprises the steps of i) forming target molecule-dependent volume-amplified entities, ii) allowing magnetic nanoparticles to bind to said volume-amplified entities, and iii) measuring changes in dynamic magnetic response of the magnetic nanoparticles caused by the increase in hydrodynamic volume of said magnetic nanoparticles.

Magnetic detection of small entities

Growing community of inventors

Uppsala, Sweden

Jenny Göransson