Becton Dickinson and Company Limited

Jennifer Tsai

Glenn K Fu

Eleen Shum

Jue Fan

Lisha Deng

Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.

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Menlo Park, CA, United States of America

Jennifer Tsai