Monsanto Technology LLC

Other

Jennifer Listello

Kevin Lee Deppermann

Phillip L Rahn

Can Duong

Angela R Cargill

Angela Ranae Koestel

R Eric Cerny

Rachel Krieb

Scott A Huber

Bernard Sammons

Amy B Martens

Jesse L Hart

Byron Froman

Kunsheng Wu

Gregory R Heck

Qingyi Zeng

John K Soteres

Cindy L Arnevik

Raymond Dobert

Philip Rahn

Kevin L Depperman

An automated seed sampler system for removing tissue from seeds includes an orientation system configured to orient seeds in a desired orientation, a sampling station configured to remove tissue from each of the oriented seeds, a sample collection subsystem configured to receive the tissue removed from each of the oriented seeds, and a seed collection subsystem configured to receive each of the seeds from which the tissue is removed. The sample collection subsystem and the seed collection subsystem are configured to facilitate a one-to-one correspondence between the seeds received at the seed collection subsystem and the tissue removed from the seeds and received at the sample collection subsystem.

Automated contamination-free seed sampler and methods of sampling, testing and bulking seeds

An automated seed sampler system includes an orientation system configured to orient a seed, and a sampling station configured to remove tissue from the oriented seed. In addition, a method for removing tissue from seeds includes positioning multiple seeds together in a desired orientation in a seed transport subsystem, and removing tissue from the oriented seeds while the seeds are in the seed transport subsystem.

An automated seed sampler system includes an imaging device for obtaining images of seeds, an orienting device for orienting the seeds based on the images, and a sampling station for removing tissue from the oriented seeds. In some aspects, the system also includes a transport subsystem for supporting the oriented seeds and conveying the oriented seeds to the sampling station. A method for removing tissue from seeds includes imaging the seeds, orienting the seeds based on image information obtained from the seeds, and removing tissue from the oriented seeds. In some aspects, the method also includes transporting the oriented seeds in a transport subsystem to a sampling station for removing the tissue from the oriented seeds, and/or collecting the tissue removed from the oriented seeds so that a one-to-one correspondence exists between the tissue and the sampled seeds, and/or analyzing the tissue for characteristics indicative of genetic and/or chemical traits.

The present invention provides transgenic soybean event MON87769, and cells, seeds, and plants comprising DNA diagnostic for the soybean event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said soybean event in a sample, methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event in a sample, growing the seeds of such soybean event into soybean plants, and breeding to produce soybean plants comprising DNA diagnostic for the soybean event.

Soybean plant and seed corresponding to transgenic event MON87769 and methods for detection thereof

A method is provided for removing tissue samples from seeds. The method generally includes orienting the seeds in a desired orientation and removing tissue samples from the oriented seeds. The method may also include orienting the seeds in the desired orientation using an actuator, where the actuator is configured to position the seed in a desired orientation. In addition, the method may include orienting the seeds together in a seed transport and removing tissue samples from the oriented seeds while the oriented seeds are in the seed transport. Further, various operations of the method may be automated as desired, for example, to permit greater sampling throughput than was previously practical.

The present invention provides a cotton plant event MON 88913 compositions and seed. Also provided are assays for detecting the presence of the cotton plant event MON 88913 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

Cotton event MON 88913 and compositions and methods for detection thereof

In various embodiments, the present disclosure provides an automated seed sampler system that includes a milling station for removing at least a portion of seed coat material from a seed and a sampling station for extracting a sample of seed material from the seed where the seed coat has been removed. A seed transport subsystem conveys the seed between the milling station and the sampling station and a seed deposit subsystem conveys the seed from the seed transport subsystem to a selected well in a seed tray after the seed has been sampled.

This invention provides a method for the development of novel plant germplasm using segregates from a transgenic line combined with PCR-based zygosity testing and, optionally, Southern blot analysis.

Growing community of inventors

O'Fallon, MO, United States of America

Jennifer Listello