Gen-probe, Inc.

Jennifer Jill Bungo

Sylvia Ann Norman

Neeraj P Rao

William L Hanna

James John Hogan

Shannon Kaplan

William G Weisburg

Michael R Reshatoff

Irene Andruszkiewicz

Reinhold B Pollner

Marie K Hudspeth

Kristin W Livezey

Xianqun Wang

Elizabeth M Marlowe

Tongjia Yin

Michael Hadjisavas

Steven T Brentano

Elizabeth M Goslow

Vivian Jonas

Steve Brentano

Shannon K Kaplan

The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid fromspeciesand/or. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.

Compositions, kits and methods for detection of campylobacter nucleic acid

The invention provides methods of determining a consensus sequence from multiple raw sequencing reads of a nucleic acid target. The nucleic acid target includes an anchor segment of known sequence and an adjacent segment of unknown sequence. The anchor segment provides a means to assess the quality of a raw target sequencing read. Raw target sequencing reads meeting or exceeding a threshold are assigned to an accepted class. The consensus sequence of the adjacent segment can be determined from raw target sequencing reads in the accepted class. Successive polling steps determine successive consensus nucleobases in a nascent sequence of the adjacent segment. Raw target sequencing reads can be removed or reintroduced from the accepted class depending on their correspondence to the most recently determined consensus nucleobase and/or the nascent sequence.

Algorithms for sequence determinations

Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.

Detection of nucleic acids from multiple types of human papillomavirus

Separation of human papillomavirus nucleic acids from biological samples

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence ofin samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.

Compositions and methods to detectnucleic acid

The present invention provides compositions, methods and kits for the species-specific detection of

Compositions, kits and related methods for the detection and/or monitoring of

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, and detection probes that hybridize specifically to23S rRNA sequences or DNA encoding 23S rRNA. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of23S rRNA sequences or DNA encoding 23S rRNA. Methods are disclosed for amplifying and/or detecting the presence ofin samples by using the disclosed compositions in in vitro methods that include nucleic acid amplification and/or detection of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product. Reaction mixtures are disclosed that contain oligonucleotides for the in vitro amplification and/or detection of23S rRNA sequences or DNA encoding 23S rRNA.

Compositions and methods to detect Legionella pneumophila nucleic acid

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San Diego, CA, United States of America

Jennifer Jill Bungo