Waters Technologies Corporation

Iowa State University Research Foundation, Inc.

Jennifer Harnisch Granger

Robert Stephen Plumb

Marc D Porter

James P Murphy

Chris Lee Stumpf

Jeffrey W Finch

Jeffery W Finch

Ananya Dubey

A separation device is formed of a first tube with a cylindrical wall enclosing a chamber having a first diameter, where the device has a first end for discharging a fluid and a second end for receiving a fluid. At least two stationary phase medias are packed into the chamber with separating frits between the medias. At least one end frit element, secured to an inner surface of the first tube contains the media in its section of the chamber and allows the beds formed of the media to be formed and packed. When the separation device so constructed forms a nanocolumn with a guard bed, the guard bed may be cleaved from the separation device extending the useful life of the nanocolumn. When the separation device so constructed comprises two analytical sections, and a plurality of guard beds, complex analysis may be performed on a column having an extended useful life.

Separation device with integral guard column

A method for making a fluid-processing apparatus involves mixing a colloidal suspension with a matrix material, applying the mixture to a surface of a conduit, and curing the applied mixture to provide a conductive coating. A fluid-processing apparatus includes a conduit and a conductive layer adjacent to a surface of the conduit. The conductive layer includes graphite particles embedded in a matrix. The fluid-processing apparatus supports, for example, electrospray-ionization for mass spectrometry, capillary electrophoresis, or capillary electro-chromatography.

Conductive conduits for chemical analyses, and methods for making such conduits

Disclosed herein are an apparatus and method for effectuating increased chromatographic efficiency in a capillary column by employing a retaining frit disposed within an analytical capillary. By disposing the retaining frit within the analytic capillary, the void volume is significantly minimized. The columns and methods described herein produce a simplified analytical capillary and retaining frit apparatus that provides greater chromatographic efficiency. Additionally, the column of the instant invention maintains chromatographic fidelity by reducing the transfer diameter as well as facilitating fluidic connections in situ.

Capillary loop with a built-in retaining frit

The invention provides nanoparticles and nanoparticle conjugates comprising one or more redox-active species, methods of making nanoparticles and nanoparticle conjugates, and methods for using nanoparticles and nanoparticle conjugates, for example, as diagnostic agents for the detection of various analytes.

Redox polymer nanoparticles

Nanocolumn chromatography devices useful, e.g., in CEC and nanoLC are disclosed. An exemplary chromatography device of the invention includes a nanocolumn, e.g., a capillary, packed with a particulate stationary phase material and a solid support. The solid support, or in situ frit, is adjacent to and integral with the stationary phase material. An in situ frit of the invention may be a mixture of the stationary phase material and a polymeric network of cross-linked poly(diorganosiloxane), e.g., poly(dimethylsiloxane), which may optionally be sintered. The invention also provides methods of making and using such devices.

Polymeric solid supports for chromatography nanocolumns

Disclosed herein is an electrospray device for mass spectrometry that includes a fluid inlet, an outlet orifice, and a passage for fluid communication between the fluid inlet and outlet orifice. This passage is formed from a capillary (i.e., a first capillary). This first capillary () partially houses a second capillary () such that the outlet orifice is narrowed. A portion () of the second capillary extends beyond the first capillary. This extension permits a practitioner to clip away obstructed portions of the second capillary.

Capillary emitter for electrospray mass spectrometry

The invention provides nanoparticles comprising one or more redox-active species, methods of making such nanoparticles, and methods for using such nanoparticles, for example, as diagnostic agents for the detection of various analytes.

Growing community of inventors

Northborough, MA, United States of America

Jennifer Harnisch Granger