Rarecyte, Inc.

Jennifer Chow

Daniel E Campton

Lance U'Ren

Steve Quarre

Joshua Nordberg

Jonathan Lundt

David Edward Stewart

Ronald C Seubert

Arturo Bernardo Ramirez

Jackie Stilwell

Edward Shafer

Elizabeth Chang

This disclosure is directed to an apparatus, system and method for introducing a liquid into a vessel. A fluid layering system includes a pump connected to a line connected to a connector, such that the connector provides an air-tight fit with the vessel.

Apparatus, system, and method for introducing a liquid into a vessel

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. An enrichment agent may be added to a vessel that contains the sample for positive selection, or, in other words, to select or aid in selecting the target material from amongst the remainder of the sample. The enrichment agent may be, for example, immunomagnetic beads, buoyant beads, high-density beads, chemicals to change the density of the target material, or the like.

Apparatus, system and method for collecting a target material

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A fraction-density-altering solution may be added to a vessel that contains the sample to change the density of a first fraction of the sample without changing the density of the target material or the density of any other sample fraction. A collector may be inserted into the vessel to funnel the target material from the sample into the collector or into a processing receptacle included in the collector. In one implementation, the collector may include a cannula which extends from a funnel into a chamber to hold the processing receptacle. In another implementation, the processing receptacle may be inserted into a bore within the collector.

Apparatus, system, and method for collecting a target material

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A first depletion agent may be added to a vessel that contains the sample to change the density of a non-target material.

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A density-altering agent may be added to a vessel that contains the sample to change the density of a non-target material.

This disclosure is directed to a slide for analyzing target analytes of a suspension. The slide can be used to collect and hold the target analyte for imaging or further processing. The slide comprises a well section, including wells into which the target analyte can be stored. The wells may be removable and sized to fit into a second apparatus, such as a PCR thermocycler, for additional processing. Alternatively, the wells may hold processing vessels, such as PCR tubes, the processing vessels being used for downstream processing and/or analysis.

Device for analysis of a target analyte

This disclosure is directed to a cap for obtaining a target analyte from a suspension. The cap introduces a magnetic field or a magnetic gradient to the tube to draw the target analyte bound to a particle to the cap. In one aspect, a cap includes a magnetic insert and a receiving piece. The magnetic insert includes a stopper and a magnet extending from the stopper; and, the receiving piece, which is configured to hold the magnetic insert, includes a receiving stopper and a sheath. The sheath may include imaging slides on opposite sides of the sheath. In another aspect, the cap may include a stopper and an embedded magnet. The cap may include an analysis piece on a bottom end of the stopper. In yet another aspect, the cap may include a fluid compartment and a filter at a bottom end of the stopper.

Device, systems and methods for analyzing a target analyte

This disclosure is directed to an apparatus, system and method for retrieving target material from a suspension. A system includes a processing vessel, such as an Eppendorf tube, a syringe or a test tube, and a collector. The collector is sized and shaped to fit into a primary vessel, such as a test tube. The collector funnels the target material from the suspension through a cannula and into the processing vessel. The cannula extends into a cavity at a first end of the collector that holds the processing vessel. The collector includes a concave opening at a second end in fluid communication with the cannula. In one implementation, the processing vessel includes at least one displacement fluid to be expelled, such that the at least one displacement fluid pushes the target material into the collector.

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Seattle, WA, United States of America

Jennifer Chow