Industrial Technology Research Institute

Personal Genomics, Inc.

Jenn-Yeh Fann

Chung-Fan Chiou

Chao-Chi Pan

Hung-Chi Chien

Hui-Ling Chen

Tsu-Ju Fu

Shang-Chia Chang

Bai-Kuang Hwang

Pei-Shin Jiang

Chih-Lung Lin

Yu-Yu Lin

Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

Method of amplifying and determining target nucleotide sequence

Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.

Methods for accurate sequence data and modified base position determination

Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.

Compositions and methods for sequencing nucleic acids

This invention is to describe nucleotide analogs used in a method for the determination of a nucleic acid sequence. The method involves an enzyme, an enzyme complex or plural number of enzymes with more than one enzymatic activity and a set of nucleotide analogs, to achieve high signal readout accuracy in nucleic acid sequencing by making each signal to have a long signaling time span which allows a higher signal clarity.

Nucleotide analogs for real-time sequencing method for single molecule

This invention is to describe a method for the determination of a nucleic acid sequence, in which it involves an enzyme, an enzyme complex or plural number of enzymes with more than one enzymatic activity and a set of nucleotide analogs, to achieve high signal readout accuracy in nucleic acid sequencing by making each signal to have a long signaling time span which allows a higher signal clarity.

Real-time sequencing method for single molecule

The invention provides a sequence calibration method, including: (a) obtaining a first reading sequence and a second reading sequence from an identical source by a receiving unit; (b) setting a comparison condition by a determining unit; and (c) comparing the first reading sequence with the second reading sequence according to the comparison condition to generate a sequence comparison result by the determining unit; and (d) outputting a calibrated sequence according to the sequence comparison result by the determining unit, wherein the comparison condition is set according to a first seed table of the first reading sequence and a second seed table of the second reading sequence.

Sequence calibration method and sequence calibration device

The invention provides a data compression method, comprising: (a) obtaining a first reading sequence and a second reading sequence from an identical source by a receiving unit; (b) comparing the first reading sequence with the second reading sequence according to a comparison condition to generate a sequence comparison result by the processor; (c) outputting a final template sequence according to the sequence comparison result by the processor; (d) comparing the final template sequence to each of the first and second reading sequences, to generate a respective difference between the final template sequence and each of the first and second reading sequences by the processor; and (e) compressing the first and second reading sequences according to the final template sequences and all generated differences between the final template sequence and the first and second reading sequences, to generate a compression file by the processor.

Growing community of inventors

Hsinchu County, Taiwan

Jenn-Yeh Fann