Syngenta Participations Ag

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Syngenta Crop Protection Ag

Jeng Shong Chen

Hope Prim Hart

Jared Conville

Yan Gao

Renshui Liu

Cheryl M Defontes

Frederick Walters

Kevin V Donohue

Richard Sessler

Guo-Qing Tang

Gregory W Warren

Vance Cary Kramer

Qiudeng Que

Milan Jucovic

Moez Rajabali Meghji

Cheryl M Stacy

Xiang Huang

Mikyong Lee

Arne Robert Anderson

Michael Kent Morgan

Henry York Steiner

Cheryl M DeFontes

Narendra Palekar

Martha M Dunn

Cheryl Marie De Fontes

Joshua Levin

Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins having toxicity to at least European corn borer are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different(Bt) Cry proteins or a modified Cry proteins a hybrid insecticidal protein having activity against at least European corn borer is designed. Nucleic acid molecules encoding the novel hybrid proteins are also provided. Methods of making the hybrid proteins and methods of using the hybrid proteins and nucleic acids encoding the hybrid proteins of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.

Insecticidal proteins

This invention provides polypeptides that were identified as interacting with Vip3 toxin. This invention also provides a method of identifying agents that bind to Vip3 interacting polypeptides, which agents may act as insecticidal agent, cytotoxic agents and/or modulate the activity of Vip3 interacting polypeptides.

Expression cassette and host cell for expressing a Vip3-interacting protein

Compositions and methods for controlling pests are disclosed. In particular, methods for modulating the activity of Cry1Ba proteins against lepidopteran or coleopteran pests are provided. Further, mutant Cry1Ba proteins having modulated insecticidal activity compared to native Cry1Ba proteins are disclosed.

Methods for modulating the activity of insecticidal proteins

Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, interfering RNA molecules that capable of interfering with pest histone genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants to confer protection from insect damage are disclosed.

Biological control of coleopteran pests

Compositions and methods for controlling plant pests are disclosed. In particular, novel engineered hybrid insecticidal proteins (eHIPs) having toxicity to at least corn rootworm are provided. By fusing unique combinations of complete or partial variable regions and conserved blocks of at least two different(Bt) Cry proteins or a modified Cry proteins an eHIP having activity against corn rootworm is designed. Nucleic acid molecules encoding the novel eHIPs are also provided. Methods of making the eHIPs and methods of using the eHIPs and nucleic acids encoding the eHIPs of the invention, for example in transgenic plants to confer protection from insect damage are also disclosed.

Improved compositions and methods for controlling pests are disclosed. In particular, nucleic acid molecules encoding novel engineered Cry1Ba (eCry1Ba) proteins having improved toxicity to lepidopteran insect pests are provided. By substituting at least one amino acid in domain I of a Cry1Ba protein an engineered Cry1Ba protein having substantially altered insecticidal properties is designed. Further, methods of making and using the ecry1Ba nucleic acid sequences, for example in transgenic plants to express eCry1B proteins to confer protection from insect damage are disclosed.

Nucleic acid molecules encoding insecticidal proteins

Engineered pesticidal polypeptides that are highly active against a wide range of pests and methods of making such polypeptides are disclosed. The nucleotide sequences encoding the pesticidal polypeptide can be used to transform various prokaryotic and eukaryotic organisms, which organisms can be used to produce the pesticidal polypeptides. The recombinant organisms and/or the polypeptides produced by the recombinant organisms can be used to control pests in various environments.

Engineered pesticidal proteins

Transgenic plants expressing a Vip3 protein have been found to be efficacious against plant-infesting nematodes. Disclosed are methods of controlling nematode populations using transgenic plants expressing Vip3 protein.

Methods and compositions for controlling nematode pests

Disclosed are double stranded RNA molecules that are toxic to coleopteran insects. In particular, dsRNA molecules that capable of interfering with pest IAP genes and that are toxic to the target pest are provided. Further, methods of making and using the interfering RNA, for example in transgenic plants to confer protection from insect damage are disclosed.

Improved compositions and methods for controlling pests are disclosed. In particular, novel engineered Cry1Ba (eCry1Ba) proteins having improved toxicity to lepidopteran insect pests are provided. By substituting at least one amino acid in domain I of a Cry1Ba protein an engineered Cry1Ba protein having substantially altered insecticidal properties is designed. Further, a method of making the engineered Cry1Ba proteins and methods of using the ecry1Ba nucleic acid sequences, for example in transgenic plants to express eCry1B proteins to confer protection from insect damage are disclosed.

Compositions and methods for controlling plant pests are disclosed. In particular, transgenic maize plants comprising nucleic acid molecules encoding modified Cry3A toxins having increased toxicity to western corn rootworm are provided. Such modified Cry3A toxins, into which a non-naturally occurring protease recognition site is inserted, are expressed at levels in transgenic plants to inhibit insect feeding damage and cause insect mortality.

Growing community of inventors

Research Triangle Park, NC, United States of America

Jeng Shong Chen