Fisons Ltd.

Hematech, LLC

Kinetic Investments Limited

Jeffrey T Wong

Xiang-Fu Wu

A method for purifying hemoglobin, mixed with other proteins is described, wherein the mixture is contacted with an ion exchange matrix so that the hemoglobin is adsorbed to it. The matrix is washed to remove unadsorbed components, and the hemoglobin is then selectively eluted using a ligand which specifically binds the hemoglobin causing it to desorb from the matrix. The method can also be used to effect reduction of methemoglobin to hemoglobin on an ion exchange matrix followed by selective elution to achieve purification. The method can also be used to purify methemoglobin.

Purification of hemoglobin and methemoglobin by bioselective elution

There is described a water soluble compound having a molecular weight of from about 70,000 to about 2,000,000 and having the formula I,

Hemoglobin complexes

There is described a water soluble compound having a molecular weight of from about 70,000 to about 2,000,000, and having the formula I,

Hemoglobin inositol phosphate compounds

Hemoglobin compounds

A blood substitute or blood extender is prepared by chemically coupling hemoglobin with a polysaccharide material selected from dextran and hydroxyethyl starch, and having a molecular weight of from about 5,000 to about 2,000,000, to form a covalently bonded chemical complex. The complex has similar oxygen transporting abilities to hemoglobin, and has a much lower rate of renal excretion.

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Don Mills, Canada

Jeffrey T Wong