Eli Lilly and Company

Jeffrey T Fayerman

Mark A Richardson

Nancy E Malin

Michael D Jones

Walter M Nakatsukasa

James A Mabe

Steven Kovacevic

James R Miller

Karl H Michel

Raymond C Yao

Charles L Hershberger

Milton J Zmijewski

Richard K Stanzak

Jeffrey L Larson

Ramamoorthy Belagaje

Haichao Zhang

Jane L Sterner

David P Greenen

Nancy E Beerman

DNA encoding porcine Pro-carboxypeptidase B, vectors comprising the DNA and host cells transformed with the vectors are useful for production of porcine carboxypeptidase B

DNA sequences encoding porcine pancreatic carboxypeptidase B

Novel vectors and methods for a single-stranded DNA mediated gene transfer system via transformation, fusion or transduction of Streptomyces, other actinomycetes, and E. coli using a variety of vectors. Phasmid shuttle vectors of the invention are particularly useful as single-stranded vectors that appear to bypass one or more host cell restriction systems, and thus increase the efficiency of gene transfer into highly restrictive host cell systems. New and useful vectors are provided that allow for the cloning of genes both for increasing the yields of known antibiotics and also for producing new antibiotics, antibiotic derivatives, or any other useful gene product, including a variety of mammalian protein products.

Methods for transforming streptomyces and other actinomycetes

Improved fermentation process for producing the Gram-positive antibiotic A47934 which comprises cultivating a new strain of Streptomyces toyocaensis, NRRL 18112, and a biologically purified culture of this microorganism are provided.

Process for producing antibiotic A47934

The present invention is a method for expressing functional polypeptides in Streptomyces using a recombinant DNA expression vector comprising a novel transcriptional- and translational- activating sequence. The novel activating sequence can be synthesized by conventional methods and used in Streptomyces expression vectors. One such vector, plasmid pFJ350, expresses and confers hygromycin resistance in Streptomyces host cells.

Method for using a novel transcriptional- and translational-activating

The present invention discloses novel recombinant DNA cloning vectors for use in Streptomyces and related organisms. These novel cloning vectors contain genetic markers that provide antibiotic resistance or colorimetric selectivity to the host cells. The invention further comprises transformants of the aforementioned vectors.

Novel cloning vectors containing selectable genetic markers for use in

The present invention discloses novel selectable recombinant DNA cloning vectors for use in Streptomyces, E. coli and related organisms. The invention further comprises transformants of the aforementioned vectors.

Novel cloning vectors for use in streptomyces, escherichia coli and

A method for expressing a functional polypeptide in Streptomyces comprises transforming a Streptomyces host cell with a recombinant DNA expression vector and then culturing the transformed cell under conditions suitable for cell growth. The recombinant DNA expression vector comprises the veg or any other homologous Bacillus promoter, a naturally occurring or modified ribosome binding site-containing DNA sequence and a gene that codes for a functional polypeptide such as human pre-proinsulin. The method is specifically exemplified by use of expression plasmids pOW529, pOW539 and transformants, Streptomyces ambofaciens/pOW529 and Streptomyces ambofaciens/pOW539. The method is broadly applicable and is particularly useful in economically important Streptomyces taxa.

Method for using an homologous bacillus promoter and associated natural

The present invention discloses selectable recombinant DNA cloning vectors for use in Streptomyces and related organisms.

Cloning vectors for use in streptomyces and related organisms

The present invention discloses multifunctional recombinant DNA cloning vectors for use in Streptomyces, Bacillus, and E. coli. The invention further discloses transformants of the aforementioned vectors.

Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and

The present invention discloses selectable recombinant DNA cloning vectors for use in Streptomyces.

Vectors for cloning in streptomyces

The present invention discloses selectable pairs of recombinant DNA cloning vectors for use in Streptomyces and related organisms.

Growing community of inventors

Indianapolis, IN, United States of America

Jeffrey T Fayerman