The Charles Stark Draper Laboratory, Inc.

The General Hospital Corporation

Massachusetts Institute of Technology

The Brigham and Women's Hospital, Inc.

Massachusetts Eye and Ear Infirmary

Other

Mobil Solar Energy Corporation

Children's Medical Center Corporation

Tufts University

The Charles Draper Laboratory, Inc.

Johnson & Johnson Innovation LLC

Jeffrey T Borenstein

Joseph L Charest

Joseph P Vacanti

Eli J Weinberg

Ernest Soonho Kim

Mark Joseph Mescher

Christopher M DiBiasio

Daniel I Harjes

Nathan Francis Moore

Vishal Tandon

Marc Steven Weinberg

Jonathan Robert Coppeta

David M Hoganson

Joseph D Cuiffi

Daniel Doty

Jason Oliver Fiering

William David Sawyer

Brett C Isenberg

Eugene A Fitzgerald

David J Carter

Michael J McKenna

Ira Spool

Anthony S Kourepenis

Robert Samuel Langer

Linda G Griffith

Amy Elizabeth Duwel

Luis Fernando Velásquez-García

Martin Nohilly

Kevin Andrew Hufford

Mitchell L Hansberry

Robert H Rubin

Ronald C Gonsiorawski

Louis Kratchman

Kenneth T Kotz

Donald Elliot Ingber

Raanan A Miller

Jonathan Jay Bernstein

Jeffrey Michael Karp

David L Kaplan

Gregory M Fritz

Joan W Miller

Christopher J Bettinger

Homer Pien

M Amin Arnaout

Gregory A Kirkos

James C Hsiao

Mohammad Reza Kaazempur-Mofrad

Abigail June Spencer

Peter Mack

Guillermo Garcia-Cardena

Alla Epshteyn

Ahmad S Khalil

James Gillett Truslow

Kevin R King

James Ching-Ming Hsiao

Samuel Walker Inman

Alexander Sevy

Jennifer Ogilvie

Ashley Lynne Beckwith

Tomoyuki Maemura

Osamu Ishii

Young-Moon Michael Shin

Chiao-Chun Wang

William Sewell

Malinda M Tupper

Jenna Leigh Balestrini

Jose A Santos

Howard I Pryor

Tatiana Kniazeva

Erin Pararas

Sarah L Tao

Kenneth C Wu

Anilkumar Harapanahalli Achyuta

Christopher E Dubé

Alla A Gimbel

Sharon G Kujawa

Else M Vedula

Ralph Hopkins

James H Connelly

Bryan D Teece

Angela B Holton

Transon Van Nguyen

Mark Laurenzi

Jonathan Wilson

Brian K Orrick

Eleanor M Pritchard

Daniel Kenneth Freeman

Stephen L Bellio

Evangelos S Gragoudas

Mathew Varghese

Andreas Zumbuehl

Anthony P Adamis

Marcello Peppi

Michael J Kardauskas

Richard D Elliott

Edwin P Chan

Chong Wing Yung

Robert Langer

Hesham Azizgolshani

Sarah Tao

A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.

Multiwell dynamic model for a tumor-immune microenvironment

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

Bi-layer multi-well cell culture platform

The present solution provides systems and methods for trans-round window membrane drug delivery. As an overview, a system can include a micropump that is connected to a flexible cannula. The cannula can be threaded through a handpiece that can be used to pierce the round window membrane of a patient. Using the handpiece, the cannula can be inserted through the round window membrane to improve the distribution of the delivered drug throughout the inner ear. The present solution can function as a small implantable or wearable device that can be used for both chronic and acute trans-round window membrane drug delivery. With this configuration, the micropump can constantly or intermittently deliver, over a period of days to months, small volumes of drugs from an internal reservoir.

Hand tool for aiding in insertion of a trans-round window membrane catheter for micropump-mediated acute and chronic inner-ear drug delivery

This disclosure describes techniques for fabricating a high-resolution, non-cytotoxic and transparent microfluidic device. A material can be selected based on having an optical property with a predetermined degree of transparency to provide viewability of a biological sample through the microfluidic device and a level of cytotoxicity within a predetermined threshold to provide viability of the biological sample within the microfluidic device. An additive manufacturing technique can be selected from a plurality of additive manufacturing techniques for fabricating the microfluidic device based on the selected material to provide a resolution of dimensions of one or more channels of the microfluidic device higher than a predetermined resolution threshold.

Systems and methods for fabricating microfluidic devices

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

Apparatus for efficient genetic modification of cells

This disclosure describes microfluidic tissue biopsy and immune response drug evaluation devices and systems. A microfluidic device can include an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample. The microfluidic device can also include a tissue trapping region at the second end of the inlet channel downstream from the first end. The tissue trapping region can include one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the tissue trap. The microfluidic device can also include one or more channels providing an outlet.

Microfluidic tissue biopsy and immune response drug evaluation devices and systems

The present disclosure discusses a system and method that includes a microfluidic device that can be used in either an extracorporeal or implantable configuration. The device supports efficient and safe removal of carbon dioxide from the blood of patients suffering from respiratory disease or injury. The microfluidic device can be a multilayer device that includes gas channels and fluid channels. Distensible membranes within the device can affect a cross-sectional area of the blood channels.

Biomimetic microfluidic device for high efficiency carbon dioxide removal from patients at low blood flow rates

Systems and methods for conducting assays on tissue fragment samples including providing a suspension maintaining pump, and a plurality of fluid reservoirs, wherein the fluid reservoirs are configured to hold a volume of fluid. The fluid reservoirs are fluidically coupled to a microfluidic assay chip, wherein the microfluidic assay chip includes a plurality of parallel assay channels, a first inlet port for introduction of a tissue fragment sample into the microfluidic assay ship, and a second inlet port coupled to the fluid reservoir. Each channel of the microfluidic assay chip also includes a retention barrier configured to trap the tissue fragment sample such that the fluid perfuses through the tissue sample, as well as an outlet port fluidically coupled to a waste receptacle.

Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs

A microfluidic device for increasing convective clearance of particles from a fluid is provided. A network of first channels can be separated from a network of second channels by a first membrane. The network of first channels can also be separated from a network of third channels by a second membrane. Fluid containing an analyte can be introduced in the network of first channels. Infusate can be introduced into the network of second channels, and waste-collecting fluid can be introduced into the network of third channels. A pressure gradient can be applied in a direction perpendicular to the direction of fluid flow in the network of first channels, such that the analyte is transported from the network of first channels into the network of third channels through the second membrane.

Systems and methods for increasing convective clearance of undesired particles in a microfluidic device

A method and system of delivering a charged cargo, such as a biomolecule, to a target structure, such as cells, exosomes, other vesicles or micelles, using an electroactive porous membrane. This method comprises contacting an electroactive porous membrane with a fluid flow toward the membrane. The fluid contains charged biomolecules and the membrane and biomolecules are oppositely charged so that the biomolecules in the fluid are trapped on the membrane as the fluid flows through the pores of the membrane. Acceptor cells of interest are pinned to the membrane by the flow of the fluid, thereby aggregating the cells onto the membrane in close proximity to the trapped biomolecules. Finally, the acceptor cells are permeabilized.

Method and system for incorporation of biomolecules into vesicles, cells, and micelles using electroactive porous device membrane

Microfluidic devices and associated methods are disclosed. A microfluidic device includes a target entrainment channel and an effluent channel on opposing sides of a semipermeable membrane. A restrictor channel that is narrower than the effluent channel is interposed between the semipermeable membrane and the effluent channel. Fluid that flows from the target entrainment channel, through the semipermeable membrane and the restrictor channel to the effluent channel, pins target cells along the center of the target entrainment channel for electroporation using an electrode in the channel.

Electroporation aided biological material delivery system and method

Growing community of inventors

Newton, MA, United States of America

Jeffrey T Borenstein