Novo Nordisk Biotech, Inc.

Paradigm Genetics, Inc.

Novo Nordisk A/s

Novozymes, Inc.

Novozymes Biotech, Inc

Metabolon, Inc.

Icoria, Inc.

Novozymes A/s

Massachusetts General Hospital Corporation

Eastern Virginia Medical School

Jeffrey R Shuster

John C Royer

Donna L Moyer

Todd DeZwaan

Lisbeth Hamer

Matthew M Tanzer

Kiichi Adachi

Sheryl Frank

Blaise Darveaux

Sanjoy Mahanty

Ryan Heiniger

Amy Skalchunes

Sze-Chung Lo

Huaqin Pan

Maria Victoria Montenegro-Chamorro

Rex Tarpey

Claus Crone Fuglsang

Sven Branner

Mark Madden

Yoder T Wendy

Randy M Berka

Michael W Rey

Ib Groth Clausen

Sakari Kauppinen

Peter Bjarke Olsen

Danny Alexander

Sven Hastrup

Tove Christensen

Klaus Peter Adam

Joshua Korzenik

Birthe Ravn Jo Slashedrgensen

Birgitte Bojer Jo Slashedrgensen

Dean A Troyer

Raymond S Lance

Garrett Zella

Rex Tarper

Maria Victoria Montenegro-Chamarro

A method of extracting and measuring one or more biochemicals from a biological sample, comprises immersing the biological sample in an organic solvent, whereby one or more biochemicals present in the biological sample are extracted into the organic solvent; separating the biological sample from the free organic solvent; and measuring the level(s) of the one or more biochemicals extracted into the organic solvent, wherein the biological sample remains analyzable by histological analysis.

Methods and reagents for metabolomics and histology in a biological sample and a kit for the same

The present invention provides various biomarkers of inflammatory bowel disease, including biomarkers for Crohn's disease and biomarkers for Ulcerative colitis. The present invention also provides various methods of using the biomarkers, including methods for diagnosis of inflammatory bowel disease, methods for distinguishing between inflammatory bowel diseases, methods of determining predisposition to inflammatory bowel disease, methods of monitoring progression/regression of inflammatory bowel disease, methods of assessing efficacy of compositions for treating inflammatory bowel disease, methods of screening compositions for activity in modulating biomarkers of inflammatory bowel disease, methods of treating inflammatory bowel disease, as well as other methods based on biomarkers of inflammatory bowel disease.

Metabolite biomarkers to distinguish Crohn's disease from ulcerative colitis and methods using the same

The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.

Morphological mutants of filamentous fungi

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing filamentous fungal expressed sequenced tags. The present invention also relates to filamentous fungal expressed sequenced tags and to computer readable media and substrates containing such expressed sequenced tags for monitoring expression of a plurality of genes in filamentous fungal cells.

Methods for monitoring multiple gene expression

The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinanthost cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.

Non-toxic non-toxigenic non-pathogenic fusarium expression system

The present inventors have discovered that Asparagine Synthase is essential for fungal pathogenicity. Specifically, the inhibition of Asparagine Synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, Asparagine Synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Asparagine Synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of asparagine synthase as antibiotics

The present inventors have discovered that Threonine synthase is essential for fungal pathogenicity. Specifically, the inhibition of Threonine synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, Threonine synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Threonine synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of threonine synthase as antibiotics

The present inventors have discovered that histidinol-phosphatase is essential for fungal pathogenicity. Specifically, the inhibition of histidinol-phosphatase gene expression in fungi results in small, non-sporulating lesions and reduced pathogenicity. Thus, histidinol-phosphatase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit histidinol-phosphatase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of histidinol-phosphate as antibiotics

The present inventors have discovered that 3-Isopropylmalate dehydratase is essential for fungal pathogenicity. Specifically, the inhibition of 3-Isopropylmalate dehydratase gene expression in fungi results in no signs of successful infection or lesions. Thus, 3-Isopropylmalate dehydratase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit 3-Isopropylmalate dehydratase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of 3-isopropylmalate dehydratase as antibiotics

The present inventors have discovered that &agr;-Aminoadipate Reductase is essential for fungal pathogenicity. Specifically, the inhibition of &agr;-Aminoadipate Reductase gene expression in fungi results in no signs of successful infection or lesions. Thus, &agr;-Aminoadipate Reductase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit &agr;-Aminoadipate Reductase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of &agr;-aminoadipate reductase as antibiotics

The present inventors have discovered that 5-Aminolevulinate synthase is essential for fungal pathogenicity. Specifically, the inhibition of 5-Aminolevulinate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, 5-Aminolevulinate synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit 5-Aminolevulinate synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of 5-aminolevulinate synthase as antibiotics

The present inventors have discovered that homocitrate synthase is essential for fungal pathogenicity. Specifically, the inhibition of homocitrate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, homocitrate synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit homocitrate synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

Methods for the identification of inhibitors of homocitrate synthase as antibiotics

Growing community of inventors

Davis, CA, United States of America

Jeffrey R Shuster