Micromass UK Limited

Jeffrey Mark Brown

Paul Robert Murray

Jason Lee Wildgoose

Martin Raymond Green

Steven Derek Pringle

Richard Denny

Emmanuelle Claude

Mark Williams Towers

Kieran Neeson

Mark McDowall

Robert Harold Bateman

Anthony James Gilbert

John Charles Gebler

Weibin Chen

Asish B Chakraborty

Steven Bajic

A method of ionising a sample is disclosed comprising nebulising a sample which includes monoclonal antibody ('mAb') molecules. A stream of monoclonal antibody droplets or charged droplets is directed so as to impact upon a target or electrode so as to form intact parent monoclonal antibody ions, intact minus light chain parent monoclonal antibody ions or light chain ('LC') fragment monoclonal antibody ions.

Method of fragmenting and charge reducing biomolecules

The present invention relates to a method of mass spectrometry, an apparatus adapted to perform the method and a mass spectrometer. More particularly, but not exclusively, the present invention relates to a method of mass spectrometry comprising the step of associating parent and fragmentation ions from a sample by measuring the parent and fragmentation ions from two or more different areas of the sample and identifying changes in the number of parent ions between the areas in the sample, and corresponding changes in the number of fragmentation ions between the two areas.

Method of mass spectrometry and a mass spectrometer

A mass spectrometer includes: an ion source; an aperture; a flight region arranged between the ion source and aperture for separating ions according to their mass to charge ratio; and ion optics arranged and configured for causing ions to be reflected or deflected while they separate according to mass to charge ratio in the flight region and such that the ions are focussed to a geometrical focal point at the aperture so that the ions are transmitted through the aperture. The multi-reflecting or multi-deflecting ion optics provides a relatively long flight path for the ions, while naturally converging the ion beam to a focus. As this focus is arranged at the aperture, it enables the aperture to be made relatively small while still maintaining high ion transmission efficiency.

Method of transmitting ions through an aperture

An imaging mass spectrometer comprising an energy source adapted to substantially simultaneously provide energy to multiple spots on a sample to produce ions from the sample by a desorption process; and an analyzer adapted to detect the arrival time and spot origin of ions resulting from said desorption process.

Imaging mass spectrometer and a method of mass spectrometry

A mass spectrometer is disclosed comprising a dual channel Solvent Assisted Inlet Ionisation ('SAII') interface.

Multi inlet for solvent assisted inlet ionisation

A mass spectrometer is disclosed comprising an Electron Transfer Dissociation cell. Positive analyte ions are fragmented into fragment ions upon colliding with singly charged negative reagent ions with the cell. The cell comprises a plurality of ring electrodes which form a spherical trapping volume. Ions experience negligible RF heating over the majority of the trapping volume which enables the kinetic energy of the analyte and reagent ions to be reduced to just above thermal temperatures. An Electron Transfer Dissociation cell having an enhanced sensitivity is thereby provided. Fragment ions created within the cell may be cooled and may be transmitted onwardly to an orthogonal acceleration Time of Flight mass analyser enabling a significant improvement in the resolution of the mass analyser to be obtained.

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Cheshire, United Kingdom

Jeffrey Mark Brown