Eli Lilly and Company

Jeffrey L Larson

Charles L Hershberger

Jeffrey T Fayerman

Haichao Zhang

Jane L Sterner

Patricia A Reynolds

David P Greenen

DNA encoding porcine Pro-carboxypeptidase B, vectors comprising the DNA and host cells transformed with the vectors are useful for production of porcine carboxypeptidase B

DNA sequences encoding porcine pancreatic carboxypeptidase B

Plasmid pHKY334, an expression vector for Met-Phe-Pro-Leu-(Asp).sub.4 -Leu-BGH, and host cells containing plasmid pHKY334 are disclosed and claimed.

Plasmid pHKY334, an expression vector for EK-BGH and host cells

The present invention discloses an .about.1.4 kb Bc1I-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.

Ultrahigh copy number streptomycetes plasmids

The present invention discloses selectable, recombinant DNA shuttle vectors for use in streptomycetes and E. coli. The shuttle vectors of the present invention are present at moderately high copy number. The invention further discloses transformants of the aforementioned vectors.

Plasmid PHJL210 and related bifunctional cloning vectors for use in

The present invention discloses chimeric plasmid SCP2 and SCP2* cloning vectors that are useful in Streptomyces and E. coli. The invention further discloses transformants and a method for detecting transformants of the aforementioned vectors.

Chimeric cloning vectors for use in streptomyces and E. Coli

A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero-specific hosts. The transformants can then be screened for clones containing genes of interest.

Growing community of inventors

Indianapolis, IN, United States of America

Jeffrey L Larson