Slingshot Biosciences, Inc.

Zymergen Inc.

Decarta Inc.

Jeffrey Kim

Anh Tuan Nguyen

Oliver Liu

Jeremy Agresti

Brandon Miller

Jennifer Shock

Keunho Ahn

Daixuan Zhang

William W Hwang

Zachariah Serber

Xiao Yang

Alexander Neckelmann

Sangita Ganesh

Michael Sneddon

Stephanie Leanne Brown Hendrix

Tom Hayon Eyles

Eyal Akiva

Samuel Oteng-Pabi

Claus Pelikan

Ute Galm

Devin Scannell

Maritza Duarte

Matt D Chalker

Christina Woo

Subhanip Biswas

Kanwal Palla

Hydrogel beads with quantifiable attached biomolecules and their use as cell mimics in cytometric applications are described. The cell mimics described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell (e.g., CD34+ stem cell). The disclosure further relates to methods of using the disclosed cell mimics as enrichment controls in cytometric applications.

CD34 stem cell mimics

Provided herein are red blood cell control compositions containing one or more populations of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter; and a population of hemoglobin molecules or a population of dye molecules that have substantially similar absorbance as hemoglobin; wherein (i) and/or (ii) are present in an amount that corresponds to a normal blood sample or disease state, or (i) and (ii) are present at a ratio that corresponds to a normal blood sample or disease state.

Engineered particles as red blood cell mimics and compositions containing same for hematology

Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

Synthetic human cell mimic particle for cytometric or coulter device

The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and/or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

Hydrogel particles as feeder cells and as synthetic antigen presenting cells

Compositions comprising multiple hydrogel particles having substantially the same diameter, but with each subgrouping of particles from the multiple hydrogel particles having different associated values for one or more passive optical properties that can be deconvoluted using cytometric instrumentation. Each hydrogel particle from the multiple hydrogel particles can be functionalized with a different biochemical or chemical target from a set of targets. A method of preparing hydrogel particles includes forming droplets and polymerizing the droplets, with optional functionalization.

Compositions and methods for passive optical barcoding for multiplexed assays

A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.

Compositions and methods for cell-like calibration particles

Synthetic human cell mimic hydrogel particle for cytometric or coulter device

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

Hydrogel particles with tunable optical properties and methods for using the same

Cytometric device hematology reference composition

The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/orAntibiotic Regulatory Protein (SARP) transcription factor.

Compositions and methods for activating silent gene clusters

Growing community of inventors

Berkeley, CA, United States of America

Jeffrey Kim