Global Life Sciences Solutions Operations UK Ltd

Ge Healthcare UK Limited

General Electric Company

Amersham Biosciences UK Limited

Qiagen Healthcare Biotechnologies Systems Gmbh

Jeffrey Kenneth Horton

Peter James Tatnell

Alan Stuart Pierce

Alison Myfanwy Wakefield

John Richard Nelson

Michael John Smith

Vincent Francis Pizzi

Simon Laurence John Stubbs

Geraint Seymour

Kathryn Louise Lamerton

Samantha Jane Ogden

Leonard Goren

Alexander Schenk

Craig Robinson

Martin David James

Samit Langar

Steven Pepe

Simon Laurence Stubbs

Michael John Gerdes

Ralf Lenigk

Cheryl Louise Potts

Ryan Charles Heller

Patrick McCoy Spooner

Suzana Kiel

Wei Sun

Rebecca Ngaire Fullerton

Paresh Lakhubhai Patel

Stephen James Capper

Christopher George Norey

Robert Stone

David Powell

Robert John

Matthew Sam Morrison

Peter Baxendale

Patrick Mccoy Spooner

Leonard J Goren

Craig Tarr Robinson

Steven Michael Pepe

Disclosed is a microfluidic device including a reagent supply apparatus comprising: a solid supportformed preferably from cellulose fibrous material having a porosity which allows liquid flow through the material; at least one generally dry reagentstored on a surface of the support at a regent location or locations; the support further being suitable for storing biological sample materialin a dry state at a sample location or locations, spaced from the reagent location or locations.

Microfluidic devices and arrangements for supplying such devices with reagents and biological samples

The present invention relates to a device and method for sample preparation and collection. More closely the invention relates to a device to isolate DNA, RNA and proteins or other biomolecules in one single step from the same undivided sample.

Device and method for sample isolation

A data storage medium is disclosed comprising a solid support matrix including an optional stabilising reagent or reagents in a dry form, for use as a support for artificially synthesised oligonucleotide sequences encoded with data. Preferably the matrix is fibrous (for example cellulose, or glass, fibres) formed into a support of sufficient strength to hold the oligonucleotide sequences. The stabilising reagents are preferably a combination of a weak base, and a chelating agent, optionally, uric acid or a urate salt, and optionally an anionic surfactant.

Oligonucleotide data storage on solid supports

Disclosed is a method for capturing the spatial molecular profiling of a biological mass formed from biological material, comprising the steps of: a) providing a transected biological mass, for example a tumour, the transection exposing at least a portion of the mass; b) providing a solid support of an area at least equalling the area of said portion of the mass; c) transferring biological material from the portion of the mass to the support to provide on the support a two dimensional imprint of the biological material present at the portion of the mass; and d) performing a biological assay of the transferred biological material from different predetermined locations of the imprint in order to determine the spatial molecular profile of the portion of the mass.

Spatial molecular profiling of solid biological masses and profile storage

A biometric device, comprising a solid support suitable for receiving biological material and for dry storing that material at room temperature the device further comprising a circuit including means for two way radio frequency communication and including writable data storage. A processing method for such biometric devices, the method including the steps of: a) identifying said device by means of RF communication; b) extracting at least a portion of the biological material from the solid support of the identified device; c) analysing the extracted biological material to obtain biometric data indicative of the biological material; and d) writing said biometric data to the data storage of the biometric device.

Biometric device and means for electronic storage and retrieval of biometric data

Embodiments of the invention provides a method for detection of at least one analyte derived from a sample, comprising the steps of: a) depositing the sample on a surface of a solid support; b) transferring at least a portion of the solid support to a receptacle suitable for performing a specific binding assay for one or more analytes of interest; c) optionally washing the portion; d) adding a single specific binding partner for each analyte of interest to the receptacle, the binding partner being labelled with an oligonucleotide sequence; e) mixing the portion with nucleic acid amplification reagents; f) amplifying the oligonucleotide sequence; and g) detecting amplified nucleic acid. The invention also provides a kit for use with the method for detection of at least one analyte derived from a sample.

Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay

Disclosed is a method for detecting and quantifying gut flora derived from human faeces, comprising acquiring a solid support containing human faeces; optionally amplifying nucleic acid from the human faeces; and detecting and quantifying the presence of gut flora of interest. Also disclosed is a method for assessing whether a person has type-2 diabetes as well as a kit for detecting and quantifying gut flora from human faeces, comprising a solid support for collecting human faeces; and primer pairs for amplifying 16S rRNA sequence from bacteria of interest.

Screening and monitoring the progression of type 2 diabetes by the molecular identification of human gut flora using FTA as a faecal collection device

Disclosed is a biological sample collectorcomprising a solid supporthaving plural discrete areas (FIG.) for accepting a biological sample, each area being chemically differentiated, for example by having a different chemical treatment sorbed onto the solid support. An envelopeencloses the solid support. A cover portioncan cover the biological sample after collection to prevent infection. UV light can be applied to the collected sample to reduce the risk of infection.

Biological sample collectors and handling thereof

The present invention relates to compositions, methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. The dried reagent composition of the invention can be used for easy processing and amplification of nucleic acid samples.

Direct nucleic acid amplification kit, reagent and method

The present invention relates to a method and kit for analyte detection. More precisely the invention relates to a method of detecting an analyte, comprising storing short single stranded nucleic acids (10-100 nt), such as aptamers and micro RNA, on a solid support at ambient temperature; and subsequent amplification of said nucleic acids for detection of analyte(s).

Method and kit for analyte detection

The present invention relates to solid supports that are used for the storage and further processing of biological materials. The invention is particularly concerned with solid supports which have at least one surface coated with a chemical that enhances the recovery of the biological material from the support. Methods of preparing and using the solid supports are also described.

Solid support and method of enhancing the recovery of biological material therefrom

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5&#x2032; end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (T) of the primer at least 10&#xb0; C. below the reaction temperature. The amplification is effected under isothermal condition.

Solid phase isothermal amplification

A microfluidic deviceis disclosed comprising a bodyincluding a biological sample S and/or reagent R receiving slot. The sample and reagent are carried on a solid support. The deviceincludes two punch assembliesfor removing portions of the solid supportand delivering them, together with the sample and/or reagent(s), to a receiving chamberfor subsequent processing. The device can be used for various assays including those which require just the addition of water in the reaction chamber with no pre-treatment of sample prior delivery into the chamber.

Microfluidic devices and arrangements for introducing reagents and biological samples to such devices

This invention relates to flat solid media for the storage of samples of biological materials and methods of analyzing biomolecules contained within the samples following storage. In particular, the invention relates to the storage and further analysis of biomolecules present in the biological materials, such as proteins, enzymes and nucleic acids. The invention finds particular utility in the dry, room temperature storage of biological materials.

Solid medium for the storage of biological material

The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

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Cardiff, United Kingdom

Jeffrey Kenneth Horton