Dna Script Sas

Roche Nimblegen, Inc.

Jeffrey Jeddeloh

Thomas Ybert

Robert Nicol

Sylvain Gariel

Xavier Godron

Adrian Horgan

Thomas J Albert

Todd Richmond

Matthew Rodesch

Jacob Kitzman

Mark D'Ascenzo

Christina Middle

The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3'-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.

Methods for extending polynucleotides

The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3'-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3&#x2032; hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.

Direct oligonucleotide synthesis on cells and biomolecules

The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.

Methods and systems for uniform enrichment of genomic regions

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Verona, WI, United States of America

Jeffrey Jeddeloh