William Marsh Rice University

Jeffrey J Tabor

Brian Landry

Nikola Dyuglyarov

Ravi Sheth

Sebastian Schmidl

Felix Ekness

Sebastian M Castillo-hair

An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into thegenome. In the absence of IPTG, Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.

expression system

Methods to create two component signal transduction systems by replace the DNA binding domains and output promoters in bacteria are described.

Identifying ligands for bacterial sensors

Growing community of inventors

Houston, TX, United States of America

Jeffrey J Tabor