Pioneer Hi-bred International, Inc.

Oregon State University

University of South Dakota

Jeffrey Habben

Dwight T Tomes

Jingrui Wu

Benjamin P Weers

Bo Shen

Nicholas J Bate

Mary A Rupe

Jeffrey R Schussler

Nathan David Coles

Shuping Jiao

Xiaomu Niu

Olga Nikolaevna Danilevskaya

Chris Zinselmeier

Christopher Zinselmeier

Timothy George Helentjaris

Huirong Gao

Jinrui Shi

Jeffrey M Hegstad

Norbert Brugiere

Kellie Reimann

Odd-Arne Olsen

Honor Renee Lafitte

Machteld C Mok

Ruth C Martin

Heather Marie Christensen

Thomas Elthon

David W S Mok

Sabrina Humbert

Olga Danilevskaya

Sarah T Collinson

Jennifer Youjin Chung

Zoran Ristic

Shailendra K Bhadula

Genping Yang

The compositions and methods relate to shorter stature corn plants having an increased and extended expression of a MADS-box polypeptide along with one or more genetic modifications that result in shorter stature.

Reduced stature maize and mads-box transcription factors

The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Genes, constructs and maize event DP-202216-6

The disclosure provides plant, plant parts, plant cells, seed and grain containing a targeted genetic modification that inserts an inverted repeat sequence into a gene. The disclosure further provides hybrid plants that contain the inverted repeat sequence in a single allele of the gene. Further provided are methods for decreasing expression of a gene encoding a polypeptide of interest in a hybrid plant by crossing a plant comprising the targeted genetic modification with a plant not comprising the genetic modification to produce a hybrid plant that contains the inverted repeat sequence in a single allele of the gene.

Dominant CRISPR inverted-repeat alleles to down-regulate gene expression in heterozygous plants

The invention provides methods for improving plant yield, particularly under nitrogen limiting conditions. According to the invention, applicants have discovered that modulating ACC synthase activity in plants improves yield of plants, even when grown under low nitrogen conditions. The same plants, while demonstrating improved yield over non-modified plants, exhibited no deleterious effects under normal nitrogen conditions. The invention further provides methods using recombinant expression cassettes, host cells and transgenic plants.

Modulation of ACC synthase improves plant yield under low nitrogen conditions

Compositions and methods for modulating flower organ development, leaf formation, phototropism, apical dominance, fruit development, initiation of roots and for increasing yield in a plant are provided. The compositions include an AP2 transcription factor sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1-11 as well as variants and fragments thereof. Nucleotide sequences encoding the AP2 transcription factors are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of an AP2 transcription factor sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising an AP2 transcription factor sequence of the invention. The level of the AP2 transcription factor polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.

Yield enhancement in plants by modulation of AP2 transcription factor

An increase in the total grain and biomass yields and photosynthetic rates of plants grown at high densities achieved by novel methods of defoliating the primary-source leaf of a majority of such plants. In particular the methods provide for increased grain and biomass yields, including for example, increased average kernel mass for maize plants, and photosynthetic rates through the timely removal of the primary-source leaf of a majority of the plants grown at high densities. This invention also relates to seeds obtained by using the disclosed methods.

Methods to increase yield of plants grown at high populations

Methods and compositions for modulating expression of knotted1 (kn1) polypeptides in seed and reproductive tissue are provided. Polynucleotide sequences encoding knotted1 transcription factor polypeptides are expressed in a spatially and/or temporally regulated manner with expression preferentially in developing flowers, pods, ears and/or seeds, or to other reproductive structures during flowering and seed development, to create a sequestered effect resulting in plants that produce increased yield and may have greater flower, pod, ear and/or seed retention. Transformed plants, plant cells, tissues and seed are also provided.

Methods and compositions for enhanced yield by targeted expression of knotted1

Methods and compositions for modulating plant development are provided. Polynucleotide sequences and amino acid sequences encoding cytokinin oxidase polypeptides are provided. The sequences can be used in a variety of methods including modulating root development, modulating floral development, modulating leaf and/or shoot development, modulating seed size and/or weight, modulating tolerance under abiotic stress, and modulating resistance to pathogens. Polynucleotides comprising CKX promoters are also provided. The promoters can be used to regulate expression of a sequence of interest. Transformed plants, plant cells, tissues, and seed are also provided.

Cytokinin oxidase sequences and methods of use

The invention discloses a transgenic method for enhancing sink strength in female reproductive organs. It involves the temporal and spatial expression of genes affecting the starch and sucrose metabolic pathways in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase yield potential over a range of environmental conditions.

Enhanced floral sink strength and increased stability of seed set in plants

This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Modulation of cytokinin activity in plants

The invention discloses a novel heat shock protein with high homology to chloroplast elongation factor EF-Tu. Also disclosed is a transgenic method for enhancing tolerance to heat and drought in female reproductive organs. It involves the temporal and spatial expression of novel heat shock EF-Tu in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase heat and drought tolerance.

Maize chloroplast protein synthesis elongation factors and methods of use for same

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise a novel nucleic acid sequence for a promoter which is induced by abiotic stress and the coding sequence natively associated with said promoter sequence. A method for expressing a heterologous nucleotide sequence in a plant using said promoter sequence is also provided. The methods comprise transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from said transformed plant cell.

Auxin-repressed, dormancy-associated promoter and uses thereof

Growing community of inventors

Urbandale, IA, United States of America

Jeffrey Habben