Agilent Technologies, Inc.

Jeffrey Fox

Holly Hogrefe

Connie Jo Hansen

Michelle H Cayouette

Weidong Wu

Bahram Arezi

Nancy Mckinney

Jennifer Lapira

Nancy McKinney

Lydia Wu

Holly H Hogrefe

Michelle Cayouette

Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3'-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

Polymerase mutants and use with 3'-OH unblocked reversible terminators

The invention provides mutants of DNA polymerases having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases.

Thermostable type-A DNA polymerase mutant with increased resistance to inhibitors in blood

The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.

Thermostable type-A DNA polymerase mutants with increased polymerization rate and resistance to inhibitors

The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.

Compositions and methods using split polymerases

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Escondido, CA, United States of America

Jeffrey Fox