Other

University of California

The General Hospital Corporation

The United States of America As Represented by the Secretary of

Commonwealth Scientific and Industrial Research Organisation

Jeffrey Ellis

Frederick M Ausubel

John M Salmeron

Brian J Staskawicz

Fumiaki Katagiri

Barbara J Baker

Guo-Liang Yu

Michael Nicholas Mindrinos

Douglas Dahlbeck

Andrew F Bent

Barbara N Kunkel

The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) expressing the candidate plant disease-resistance gene within the plant tissue sample; and (d) determining whether the plant tissue sample exhibits a disease-resistance response, whereby a response identifies a plant disease-resistance gene.

Methods of identifying plant disease-resistance genes

Disclosed is substantially pure DNA encoding an,Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

RPS gene family, primers, probes, and detection methods

Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

Growing community of inventors

Weetangera, ACT 2614, Australia

Jeffrey Ellis