Hoffmann-la Roche Inc.

American Cyanamid Company

Jeffrey David Hulmes

Bradley Alton Ozenberger

Ulrich Andreas Gubler

Maurice Kent Gately

Alvin Seth Stern

Richard Anthony Chizzonite

Yu-Ching Eugene Pan

Frank John Podlaski

Cecil Mark Eppler

C Mark Eppler

The present invention is a novel cytokine protein called IL-12 or Cytotoxic Lymphocyte Maturation Factor (CLMF) which is produced and synthesized by human NC-37 B lymphoblastoid cells (American Type Culture Collection, Rockville, Md.). CLMF synergistically induces with low concentrations of IL-2 the cytolytic activity of Lymphokine Activated Killer (LAK) cells, and CLMF is capable of stimulating T-cell growth. Also claimed are the cloned gene for CLMF, its recombination in a suitable vector, the transformed cells containing said vector, the recombinant protein produced by the transformed cells and antibodies to CLMF.

Purification and characterization of cytotoxic lymphocyte maturation factor and monoclonal antibodies thereto

cDNAs encoding proteins closely related to opioid receptors

Isolated DNAs encoding opiorph receptor polypeptides are provided. Recombinant cloning vectors which include these DNA sequences and cells which include these vectors are also provided. Methods for detecting the expression, in a tissue, of mRNA encoding a polypeptide encoded by this DNA are encompassed as well as methods for producing these polypeptides. These isolated polypeptides and antibodies to these polypeptides are also contemplated.

Proteins closely related to opioid receptors

Method of detecting expression of proteins closely related to opioid

The present invention relates to antibodies which bind to a novel cytotoxic lymphocyte maturation factor. When bound to the cytotoxic lymphocyte maturation factor, the antibodies can neutralize bioactivity of the factor.

Monoclonal antibodies to cytotoxic lymphocyte maturation factor

Growing community of inventors

Ringwood, NJ, United States of America

Jeffrey David Hulmes