Zymogenetics, Inc.

Baxter International Inc.

Mallinckrodt Pharma IP Trading D.a.c.

Jeffrey D Meyer

Richard I Senderoff

Paul D Bishop

Steven D Hughes

Gerald W Lasser

Richard M Garcia

Emily N Rollins

Shan Jiang

Chung Chan

Hong Yan Liu

Bruce L Zamost

Susan D Holderman

Karen S De Jongh

Douglas C Covert

Provided herein are hemostatic compositions. In one embodiment, the hemostatic composition includes cross-linked polymer microspheres, such as cross-linked gelatin microspheres with pores. In another embodiment, the hemostatic composition comprises an additive such as a wetting agent, a suspending agent, or both. The hemostatic compositions may also include a hemostatic agent such as thrombin, and may include a high concentration of thrombin. The hemostatic compositions may also include plasma. Also provided herein are devices for dispersing said hemostatic compositions in a diluent, and delivering said dispersed hemostatic composition. The hemostatic compositions may also fabricated with a selected geometry as administration suggests.

Hemostatic microspheres

The expression vectors and methods using anexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Using the expression vectors, the IL-21 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains transformed with an IL-21 expression vector.

Compositions of IL-21 produced in prokaryotic hosts

Compositions comprising thrombin and methods of preparing them are disclosed. The composition may be in the form of an aqueous solution at pH 5.7-7.4 consisting essentially of 0.1 mg/mL to 5.0 mg/mL thrombin, 2% to 4% (w/v) sucrose, 3.5% to 5% (w/v) mannitol, 50 mM to 300 mM NaCl, 0-5 mM CaCl, 0.03% to 1% (w/v) of a surfactant or high-molecular-weight polyethylene glycol, and a physiologically acceptable buffer, or may be in lyophilized form.

Thrombin compositions

The expression vectors and methods using anexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation inUsing the expression vectors, the IL-21 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains transformed with an IL-21 expression vector.

IL-21 production in prokaryotic hosts

Growing community of inventors

Lake Forest Park, WA, United States of America

Jeffrey D Meyer