Genencor International, Inc.

Niro A/s

Henry G. Heinsohn

Jeffrey D. Lorch

Kirk J. Hayenga

Raymond E. Arnold

Jeffrey D Lorch

Kathleen A Clarkson

Edmund A Larenas

Geoffrey L Weiss

Michael C Ward

Kirk James Hayenga

Henry G Heinsohn

Raymond E Arnold

Benjamin S Bower

David A Johnson

The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.

Trichoderma longibrachiatum EGIII cellulase

DNA sequence encoding endoglucanase III cellulase

A process for treating waste water produced in a cheesemaking operation. The process comprises treating a milk product so as to form a curd, subjecting the curd to an elevated temperature while in contact with water and separating the curd from a water-containing material including milk fats. The process further includes subjecting the water-containing material to microfiltration in a microfilter so as to produce a concentrate rich in the milk fats and a permeate, and recovering the concentrate and the permeate.

Process of treating water containing effluent from cheese-making

Methods for isolating EG III cellulase component and enriched EG III cellulase in polyethylene glycol are disclosed. The methods comprise adding an inorganic salt and polyethylene glycol having a molecular weight of from about 5,000 to 10,000 to an aqueous mixture of cellulase proteins under conditions to create a two-phase system. Next, the cellulase proteins other than EG III are separated in an EG III-poor aqueous phase while EG III cellulase component is retained in an EG III-rich polyethylene glycol phase. Lastly, the EG III component can be separated from the polyethylene glycol. The preferred method of separation is alcohol precipitation.

Methods for isolating EG III cellulase component and EG III cellulase in

Disclosed are methods for the recovery and purification of naturally produced chymosin. In particular, disclosed are methods for the recovery and purification of chymosin from aqueous solutions containing chymosin, pepsin and other contaminants.

Processes for the recovery of naturally produced chymosin

Disclosed are methods for the recovery of microbially produced chymosin. In particular, disclosed are methods for the recovery of chymosin from the fermentation beer arising from culturing microorganisms which have been engineered so as to produce chymosin. Also disclosed are methods for the selective recovery and subsequent purification of microbially produced chymosin.

Processes for the recovery of microbially produced chymosin

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Hudson, WI, United States of America

Jeffrey D Lorch