Gen-probe, Inc.

Jeffrey D Chismar

James M Carrick

Mark R Kennedy

Michael J Gilly

System for quantifying analyte polynucleotides employs computer-implemented analysis of real-time amplification data using a calibration curve defined by parametric equations.

Parametric system for quantifying analyte polynucleotides

System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives.

Real-time amplification and monitoring system incorporating vector-based algorithm for analyzing polynucleotide-containing samples

Method of preparing a calibration curve and determining the amount of an analyte polynucleotide present in a test sample, particularly using real-time amplification data. The method involves determining indicia of amplification for both the internal calibrator and analyte polynucleotide standards, each as a function of the amount of analyte polynucleotide standard input into the amplification reaction.

Parametric calibration method

Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

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Plymouth, MN, United States of America

Jeffrey D Chismar