Bio-rad Laboratories, Inc.

Raindance Technologies, Inc.

Variagenics, Inc.

Sequenom, Inc.

Nuvelo, Inc.

Jeffrey Charles Olson

Darren Roy Link

Michael L Samuels

Keith Brown

Andrew Watson

Vincent P Stanton, Jr

Jia Liu Wolfe

Martin Zillmann

John Brian Hutchison

Gregory Lawrence Verdine

Tomohiko Kawate

Vince P Stanton, Jr

Colin W Dykes

Martin Zillman

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Compositions and methods for molecular labeling

In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.

Digital analysis of nucleic acid modification

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

Digital analyte analysis

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Methods for determining genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information.

Methods for genetic analysis of DNA to detect sequence variances

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Chelmsford, MA, United States of America

Jeffrey Charles Olson