Synageva Biopharma Corporation

University of Georgia Research Foundation, Inc.

Avigenics, Inc.

University of Iowa Research Foundation

Alexion Pharmaceuticals, Inc.

Avigentics, Inc.

Jeffrey C Rapp

Alex J Harvey

Robert D Ivarie

Julie A Morris

Guodong Liu

Leandro Christmann

Markley C Leavitt

Dawn M Eberhardt

Stephen H Parker

Scott L Pratt

A transgenic avian containing in its genome an exogenous nucleotide sequence which includes a promoter component and a vector with reduced promoter interference wherein the exogenous nucleotide sequence is integrated into the genome and the avian.

Method of using a transgenic chicken to produce exogenous proteins in its eggs

The invention includes isolating pharmaceutical proteins from avian hard shell eggs containing pharmaceutical proteins wherein the pharmaceutical proteins are exogenous to the egg.

Method of making protein in an egg of a transgenic chicken

This invention provides for proteins which are expressed in the avian oviduct, packaged into eggs laid by the avian, then isolated.

Production of exogenous proteins in egg whites of transgenic chickens

This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.

Transgenic chickens producing exogenous protein in eggs

Nucleic acid construct having an ovalbumin promotor

The invention provides for antibodies produced in transgenic birds as chickens.

Antibodies produced in a transgenic avian egg white

This invention provides for proteins which are expressed in the avian oviduct, packaged into eggs laid by the avian and removed from the eggs.

Method of producing an exogenous protein in the egg of a chicken

The invention relates to G-CSF molecules having certain O-linked oligosaccharide structures.

Glycosylated human G-CSF

The invention relates to interferon-&#x3b1; molecules having certain O-linked oligosaccharide structures.

Glycosylated interferon alpha

This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

Production of a transgenic avian by cytoplasmic injection

The invention provides for transgenic avians containing nucleic acids which include an exogenous lysozyme gene expression controlling nucleotide sequence which typically is linked to a polynucleotide encoding a heterologous polypeptide.

Avians containing a lysozyme promoter transgene

This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.

Avians expressing heterologous protein

Granulocyte colony stimulating factor obtained from eggs laid by transgenic avians having newly described G-CSF glycosylation patterns.

Glycosylated G-CSF obtained from a transgenic chicken

This invention provides for human interferon-&#x3b1; which includes novel glycosylation structures.

Glycosylated interferon &#x3b1;

The present invention relates to cells that can be passaged in culture and can be used for, among other things, promoter assays and the production of heterologous proteins.

Growing community of inventors

Athens, GA, United States of America

Jeffrey C Rapp