Wisconsin Alumni Research Foundation

Other

Jeffrey C Mohr

Sean Paul Palecek

Nicholas Lawrence Abbott

Christopher John Murphy

Nathan Lockwood

Juan J Depablo

Elise Huang

Michael R Mohr

Juan Jose De Pablo

Timothy Joseph Kamp

Jianhua Zhang

A system and method are presented that store graph data in a database as nodes, edges that link nodes, and detached profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Detached profiles allow a single profile, comprising a set of attribute-value pairs, to be associated with multiple database objects, or to survive the deletion of associated objects.

Method and system for dynamically creating and exploring graph structures

A system and method are presented that store graph data in a database as nodes, edges that link nodes, and profiles that contain properties that provide additional context for the nodes and edges. Computerized methods are described that provide for the dynamic creation of graph data based on the properties stored on the profiles related to each node, including the ability to predetermine which properties are used within a CSS-like language. Methods for visually focusing on a portion of a graph are also disclosed that allow a user to select one or more base nodes and limit the display of related nodes to those nodes within a defined degree of separation from the base node(s). The user can control the desired degree of separation, can select multiple base nodes, and can predetermine the base nodes to be used (and desired degree of separation for each) using a CSS-like language.

A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel&#xae;-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.

Method for culturing stem cells

The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

Liquid crystalline substrates for culturing cells

Growing community of inventors

Madison, WI, United States of America

Jeffrey C Mohr