Dow Agrosciences LLC

Jeffrey Beringer

Manju Gupta

Navin Elango

Sara Bennett

Karthik Narayna Muthuraman

Huixia Wu

Paul R Schmitzer

Sandeep Kumar

William M Ainley

Frank Burroughs

Narasimha Chary Samboju

Robbi J Garrison

Nagesh Sardesai

P Samuel Jayakumar

Joseph F Petolino

Andrew F Worden

Raghav Ram

Asha Mehra Palta

Bridget D Kosegi

Shavell Gorman

Karthik N Muthuraman

Andrew Worden

This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3'UTR fromchlorophyll a/b binding protein gene. Some embodiments relate to a 3&#x2032; UTR from achlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3' UTR fromchlorophyl A/B binding protein gene

Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5'-UTRs, and/or 3&#x2032;-UTRs, isolated from

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Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5'-UTRs, introns, and/or 3&#x2032;-UTRs, isolated from

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues usingmetallothionein-like gene regulatory elements.

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3'-UTRs, isolated fromubiquitin genes.

Constructs for expressing transgenes using regulatory elements from brachypodium ubiquitin genes

Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues usingchlorophyll a/b binding gene regulatory elements.

The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls

In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls, and transformation thereof

Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called 'whiskers'. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is facilitated.

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Carmel, IN, United States of America

Jeffrey Beringer