University of Medicine and Dentistry of New Jersey

Rutgers, The State University of New Jersey

Kane Biotech Inc.

Jeffrey B Kaplan

Srinivasa Madhyastha

Karen Lovetri

Scott Kachlany

Nandadeva Yakandawala

Purushottam V Gawande

Evguenii Vinogradov

Nanda Yakandawala

Daniel Rhoads

Lasha Gogokhia

Purusbottam Gawande

Nataliya V Balashova

Polysaccharide-containing extracts isolated from a host cell containing nucleotide sequences encoding genes pamA, pamB and pamC, wherein the extract is capable of inhibiting biofilm formation produced by gram-negative bacteria, gram-positive bacteria and fungi, and methods to inhibit biofilm formation or remove biofilms that have already formed.

Biofilm inhibiting composition

The present invention provides compositions comprising an antibiofilm enzyme, a soluble &#x3b2;-N-acetylglucosaminidase similar to the dspB gene (DispersinB&#xae;), and an antimicrobial for preventing growth and proliferation of biofilm-embedded microorganisms in acute and chronic wounds, and methods of treatment. The invention further provides methods for preparing medical devices, and in particular, wound care devices using soluble &#x3b2;-N-acetylglucosaminidase based antimicrobial compositions.

Soluble &#x3b2;-N-acetylglucosaminidase based antibiofilm compositions and uses thereof

The present invention relates to antimicrobial deoxyribonuclease-based compositions that inhibit growth and proliferation ofbacteria. The present invention also relates to methods of administering the compositions in the treatment and prevention ofinfections. The present invention also relates to methods of administering the compositions in the eradication ofnasal carriage, in order to prevent the transmission ofbacteria.

[object Object]

The present invention provides antibiofilm composition comprising two or more agents selected from the group consisting of DispersinB&#x2122;, 5-Fluorouracil, Deoxyribonuclease I and Proteinase K for preventing growth and proliferation of biofilm-embedded microorganisms in wound care, oral care, and disease-related infections and methods of treatment in mammals. The invention further provides methods for preparing medical devices, and wound care devices using an antibiofilm composition comprising two or more antimicrobial agents selected from the group consisting of DispersinB&#x2122;, 5-Fluorouracil, Deoxyribonuclease I and Proteinase K.

DispersinB&#x2122;, 5-fluorouracil, deoxyribonuclease I and proteinase K-based antibiofilm compositions and uses thereof

Isolated nucleic acid sequences and amino acid sequences for soluble, &#x3b2;-N-acetylglucosaminidase or active fragments or variants thereof which promote detachment of bacterial cells from a biofilm are provided. An isolated mutant bacteria which forms biofilm colonies which tightly adhere to surface but which are unable to release cells into the medium or spread over the surface is also provided. In additions, methods are described for modulating detachment of bacterial cells from biofilm by mutating soluble, &#x3b2;-N-acetylglucosaminidase or altering its expression or activity are also provided. Also provided are compositions, methods and devices for preventing, inhibiting and treating bacterial infections.

Dispersin B polypeptides and uses thereof

Dispersin B polynucleotides and methods of producing recombinant DspB polypeptides

Compositions and methods for enzymatic detachment of bacterial and fungal biofilms

Isolated nucleic acid sequences and amino acid sequences for soluble, &#x3b2;-N-acetylglucosaminidase or active fragments or variants thereof which promote detachment of bacterial cells from a biofilm are provided. An isolated mutant bacteria which forms biofilm colonies which tightly adhere to surface but which are unable to release cells into the medium or spread over the surface is also provided. In additions, methods are described for modulating detachment of bacterial cells from biofilm by mutating soluble,&#x3b2;-N-acetylglucosamimidase or altering its expression or activity are also provided. Also provided are compositions, methods and devices for preventing, inhibiting and treating bacterial infections.

Growing community of inventors

Monsey, NY, United States of America

Jeffrey B Kaplan