Gelman Sciences, Inc.

Jeffrey Ambler

In accordance with the invention the glycosylated hemoglobin in a blood sample is electrophoretically separated from the other constituents by including dextran sulphate in the electrophoresis buffer, or by mixing a solution of the dextran sulphate with the blood sample prior to the electrophoresis, such that the blood is in contact with dextran sulphate during the electrophoresis. The presence of the dextran sulphate results in a clear and well defined electrophoretic separation of the glycosylated hemoglobin from the non-glycosylated hemoglobin, thereby enabling accurate glycosylated hemoglobin determination.

Electrophoresis method for detecting glycosylated hemoglobin in blood

A non-barbiturate buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of a water soluble salicylate, a water soluble inorganic salt such as sodium chloride, and buffer to maintain the pH at from about 8.2 to 9.0.

Buffer composition and method for the electrophoretic separation of

A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R.sub.1 --CO--NH--R.sub.2 --COOH where R.sub.1 is NH.sub.2 or an alkyl or aryl group, preferably NH.sub.2 , C.sub.6 H.sub.5 or C.sub.6 H.sub.4 NH.sub.2 and where R.sub.2 is an alkyl group, preferably CH.sub.2, CH.sub.2 --CH.sub.2 or CH(CH.sub.3); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2 to 9.0. The preferred acid is hippuric acid.

A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R.sub.1 --CO--NH--R.sub.2 --COOH where R.sub.1 is NH.sub.2 or an alkyl or aryl group, preferably NH.sub.2, C.sub.6 H.sub.5 or C.sub.6 H.sub.4 NH.sub.2 and where R.sub.2 is an alkyl group, preferably CH.sub.2, CH.sub.2 --CH.sub.2 or CH(CH.sub.3); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2 to 9.0. The preferred acid is hippuric acid.

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Tollerton, United Kingdom

Jeffrey Ambler