Dyax Corporation

Biotage, Inc.

Biotage Ab

Jeffrey A Horsman

Peter C Van Davelaar

Peter C Rahn

Ivan Hargro

Peter J Leavesley

Robert D Lockman

John R Bickler

Kelvin J Hammond

Shahnaz Ghassemi Jamalabadi

Omar Mneimne

Thomas R Schumacher

Ivan Hargo

William Schnoebelen

Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Control system and method for flash separation

A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy.

Processing of chemicals in flow-through device with porous media

An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.

Chromatography apparatus

Apparatus for chromatography, featuring a composite chromatography column including a tubular member having an inlet end and an outlet end, where the tubular member includes an innermost first layer comprised of a first material and a second layer comprised of a second material. The chromatography apparatus can also include a first intermediate layer formed from a third material, where at least a portion of the first intermediate layer is disposed between the innermost first layer and the second layer. A second intermediate layer formed from a fourth material, can further be included in the chromatography apparatus, where at least a portion of the second intermediate layer is disposed between the innermost first layer and the second layer. A portion of the second intermediate layer can be disposed between the second layer and the first intermediate layer.

Composite chromatography column

A method of processing a sample comprising introducing a sample into a flow-through device containing a porous solid media therein, and thereafter subjecting the device to microwave energy. Also disclosed is a flow through device including solid porous media therein and attached active components.

A device for transferring small drops of liquid from one or more liquid holders to one or more liquid destinations is disclosed. The device includes a support member and a plurality of fluid transfer members extending from the support member. Each fluid transfer member includes a flexible liquid transfer end.

Liquid transfer device

An apparatus for use in liquid chromatography involves a column filled with media bounded axially by at least one porous member. The column has an inlet end with an inflow region between the inlet and a porous member. The inflow region may be sufficiently long so that a chromatography sample module may be disposed therein. The column may be sufficiently long so that a chromatography sample module may be disposed therein. The column may also have an outlet end with an outflow region between the outlet end and a second porous member. The inflow region may be substantially longer than the outflow region. The porous members may be moveable relative to the column.

A chromatography column includes a tubular member with an inlet end and a slidable porous member that bounds a chromatography media. The porous member is spaced sufficiently from the inlet end to define a receiving region.

Chromatography column

A chromatography sample module including a flow-through member having an inlet and an outlet, chromatography media within the flow-through member, and a sample carried on the media. The module can fit within a chromatography column, and a plurality of modules can be arranged in an array in a rack to facilitate processing of multiple samples.

Module and method for introducing a sample into a chromatography column

A check valve module includes a body having a first fluid communicating surface and a second fluid communicating surface, and two one-way check valves in the body controlling flow between the first and second surfaces. One of the valves permits flow from the first surface to the second surface, and the other valve permits flow from the second surface to the first surface. The body also has an outer sealing surface between the first and second fluid communicating surfaces.

Check valve module

A pump drive assembly is disclosed. The assembly includes a driver, a pump drive member driven by the driver, and a decoupler that decouples the drive member from the driver when a force acting upon the drive member exceeds a threshold force. The pump drive member can be a piston rod, and the decoupler can be a magnet attached to the piston rod and coupled to a magnetically attracted plate driven by the driver. When a force resisting movement of the piston rod exceeds the magnetic force coupling the magnet to the plate, the magnet decouples from the plate, and the pumping operation ceases.

Pump drive decoupler

Apparatus for simultaneously supplying solvent to a plurality of chromatography columns using a plurality of pumps. Each pump has a chamber with a movable solvent piston therein and inflow and outflow valves. The outflow valve of each pump is connected to a respective chromatography column. The solvent pistons are simultaneously moved in one direction to pull solvent into the respective chambers until all pistons have achieved an adjustable stop position corresponding to a desired delivery volume. The solvent pistons are then simultaneously moved in the opposite direction to deliver solvent to the respective columns until all pistons have delivered the desired delivery volumes. The solvent pistons are driven by a common pneumatic or hydraulic system.

Growing community of inventors

Charlottesville, VA, United States of America

Jeffrey A Horsman