Emulate, Inc.

Harvard College

Cedars-sinai Medical Center

Jefferson Puerta

Daniel Levner

Norman Wen

S Jordan Kerns

Christopher David Hinojosa

Geraldine A Hamilton

Josiah Daniel Sliz

Clive N Svendsen

Robert N Barrett

Jacob Fraser

Dhruv Sareen

Magdalena Kasendra

Carol Lucchesi

Stephen R Targan

Michael Workman

Uthra Rajamani

David Conegliano

Brian Zuckerman

Tanvi Shroff

Grace Ahn

Jonathan Rubins

Victor Antontsev

Donald Elliot Ingber

Chris Hinojosa

Richard Novak

Miles Ingram

Tiama Hamkins-Indik

David James Coon

Daniel E Shea

Jordan Kerns

Magdelena Kasendra

James Coon

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Compound distribution in microfluidic devices

A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.

Method for micromolding a polymeric membrane having a pore array

This invention is in the field of surface modification. In particular, the invention relates to the surface modification of microfluidic devices to alter surface hydrophobicity characteristics.

Surface functionalization

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Malden, MA, United States of America

Jefferson Puerta