Third Wave Technologies, Inc.

Honeywell International Inc.

Northwestern University

Gen-probe, Inc.

Jeff G Hall

Victor I Lyamichev

Bruce P Neri

James Robert Prudent

Mary Ann D Brow

James E Dahlberg

Robert W Kwiatkowski, Jr

Andrew A Lukowiak

Andrea L Mast

Hatim T Allawi

Zbigniev Skrzypczynski

Tsetska Y Takova

Lloyd M Smith

Peggy S Eis

Michael W Kaiser

Mary Ann Brow

Sarah Olson

Marilyn C Olson-Munoz

LuAnne Chehak

Hon Ip

Christian Tor Bartholomay

Michelle Curtis

James J Schaefer

WuPo Ma

Kevin L Vedvik

Lin Ji

Natalie E Lyamicheva

Lisa C Thompson

Sarah R Wayland

David M Kelso

Joseph J King

Michael Graham

Soumitri N Kolavennu

Andy M Peczalski

David J Marshall

Brian Aizenstein

Mark A Mantua

Robert W Kwiatkowski

Poonam Agarwal

Scott M Law

Patrick Lynn Peterson

Brad T Argue

David Michael Olive

Stephanie H Vavra

Priscilla Wilkins Stevens

Patrick Peterson

Natalie E Lyamichev

Sarah M Olson

Wupo Ma

Hon S Ip

Hatim Taysir Allawi

Joerg Werner Baier

Darryl Ballard

Todd Hanson

Ned D Reimer

Susan S Wigdal

Luanne Chehak

Michelle L Curtis

Zbigniew Skrzypczynski

James L Dahlberg

Jennifer M Schneiders

Mary C Oldenberg

Carolyn W Miller

Deborah E Burris

Monika De Arruda Indig

Nancy Jarvis

Luis P Reynaldo

Vincent Tevere

Natalie Lyamichev

Eric B Rasmussen

David Arco

Myrta W Atiles

Molly A Accola

Laura Heisler

Bruce N Neri

Jr Robert W Kwiatkowski

Natascha Lyamicheva

Joe King

Disclosed are methods for diagnosing Bacterial Vaginosis (BV). The disclosed methods generally include detecting select species ofand/or, and optionally detecting select species of. Also disclosed are nucleic acid oligomers and related compositions for detection of a 16S rRNA or its encoding gene from select species of, or

Methods for diagnosing bacterial vaginosis

Reaction mixtures for diagnosing bacterial vaginosis

A switch system includes a snap action switch, an operating plunger, and an actuator. The snap action switch is configured to move, with snap-action, from a first switch position to a second switch position. The operating plunger is disposed adjacent to the snap action switch and is coupled to selectively receive an actuating force. The operating plunger is configured, upon receipt of the actuating force, to retain the snap action switch in the first switch position. The operating plunger is further configured, upon removal of the actuating force, to allow the switch to move from the first switch position to the second switch position. The actuator contacts the operating plunger and is configured to selectively supply the actuating force to, and remove the actuating force from, the operating plunger. The operating plunger comprises a dielectric material having low thermal conductivity.

Switch system with high temperature operating plunger

The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3' hairpin structures, 5&#x2032; hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5&#x2032; or 3&#x2032; ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.

Snap-back primers and detectable hairpin structures

A redundant non-contact switch reports a status as closed or open for a first member and a second member that move relative to each other between an open state and a closed state. In some embodiments, the redundant non-contact switch includes a wireless authentication (WA) pair and a magnetic pair. The WA pair may include a WA responder attached to one of the first member and the second member, and a WA interrogator attached to the other of the first member and the second member. The WA pair may be configured to register a WA status of closed or open, depending on a WA authentication between the WA responder and the WA interrogator. The magnetic pair may include a magnet attached to one of the first member and the second member, and a magnet sensor attached to the other of the first member and the second member. The magnetic pair may be configured to register a magnetic status of closed or open, depending on whether a magnet distance between the magnet and magnet sensor is beyond a threshold magnet distance. In some instances, the redundant non-contact switch reports the status as closed only if both the WA status is registered as closed and the magnetic status is registered as closed.

Secure non-contact switch

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

Detection of nucleic acids by multiple sequential invasive cleavages

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

RNA detection assays

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Cleavage of nucleic acids

The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5' nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Reactions on a solid surface

The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.

Charge tags and the separation of nucleic acid molecules

Invasive cleavage of nucleic acids

The present invention provides compositions comprising oligonucleotides that have 3' end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3&#x2032; end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.

3' end tagged oligonucleotides

Growing community of inventors

Madison, WI, United States of America

Jeff G Hall