USA as Represented by Secretary of the Navy

Geo-Centers, Inc.

Other

Jeff Calvert

Lisa C Shriver-Lake

Jacque Georger

Frances S Ligler

Charles S Dulcey

Suresh Bhatia

Reinhard Bredehorst

Suresh K Bhatia

Frances S Eigler

Irradiating, with ultraviolet light, surfaces which contain thiol groups, epoxy groups, or vicinal diol groups, results in surfaces which exhibit a reduced adsorption of biomolecules. In the case of surfaces having thiol groups such irradiation also results in a reduced capacity for the bonding of heterobifunctional crosslinking reagents. Such irradiation may be carried out in a patternwise fashion to obtain patterned surfaces.

Method for surface modification to create regions resistant to

Surface modification to create regions resistant to adsorption of

Active agents such as proteins are covalently immobilized on substrates carrying hydroxyl groups. A silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality. Preferably, the silane has a functional group which reacts with the hydroxyl group of the substrate and a thiol terminal group which reacts with a functional group of a heterobifunctional crosslinking agent which contains a succinimide group that reacts with an amino group of the active agent. Bound active agents such as proteins are useful as biosensors or reactants in a variety of applications including bioassays.

Immobilization of active agents on substrates with a silane and

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Burke, VA, United States of America

Jeff Calvert