Institut Francais Du Pe'trole

Institut Fran.cedilla.ais Du Petrole

Jeannine Lemal

Remy Marchal

Caroline Sulzer

Anne-Marie Davila

A process for the fed batch production of a sophorolipid composition is described, in which culturing takes place of at least one Candida bombicola strain in a culture medium incorporating a sugar and a nitrogen source and said cultured strain is exposed in a reaction zone to a supply of an appropriate substrate under adequate aeration, temperature and pH conditions and at least once the following sequence is performed: a) the strain is continuously supplied with substrate at a supply rate in the reaction zone between 0.01 and 4 grams per hour and per liter of initial reaction volume and for a supply time such that the residual substrate concentration in the reaction zone is maintained at a value at the most equal to 18 grams per liter of initial reaction volume during said supply time; and b) the sophorolipid composition produced is recovered; the process being characterized in that the substrate essentially consists of at least one animal oil, at least one vegetable and/or at least one ester of said oil, said oils and said ester incorporating an aliphatic linear chain with 10 to 24 carbon atoms.

Growing community of inventors

Rueil Malmaison, France

Jeannine Lemal