Luxembourg Institute of Science and Technology

Luxembourg Institute of Health (lih)

Jean-Sebastien Thomann

Gaelle Corne

Damien Lenoble

Valerie Palissot

A method for manufacturing supported lipid bilayer on a porous silica nanoparticle with a &#x3b6;-potential comprised between &#x2212;10 mV and +10 mV, the method comprising the steps of (a) providing a negatively charged supported lipid bilayer on a porous silica nanoparticle, wherein the negatively charged supported lipid bilayer has a &#x3b6;-potential inferior to &#x2212;15 mV and wherein the negatively charged supported lipid bilayer comprised at least one phospholipid and; (b) adding a formulation of lipids, the lipids being 1,2-dioleoyl-3-trimethylammonium-propane alias DOTAP, cholesterol and at least one lipid different from DOTAP and cholesterol. The method further comprises the step of (c) performing an ultra-sonication for promoting DOTAP incorporation. The method can be supplemented by the step of addition of alginate and the step of cross-linking the alginate. Also a nanocapsule and composition comprising the nanocapsule.

Nanocapsules and method for manufacturing thereof

A method for manufacturing a negatively charged supported lipid bilayer. The method comprises the steps of preparing a formulation comprising at least three lipids (1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS), cholesterol and at least one lipid different from DOPS and cholesterol) dissolved in a first solvent, of evaporating the first solvent, of adding an aqueous formulation of mesoporous silica nanoparticles, of performing an ultra-sonication and of performing a centrifugation. The method is remarkable in that the number of equivalents of cholesterol relative to one equivalent of DOPS is comprised between 2.30 and 2.70. Additionally, negatively charged supported lipid bilayer on a mesoporous silica nanoparticle comprising cholesterol, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and at least one lipid different from DOPS and cholesterol.

Negatively charged self-assembling supported lipid bilayer on mesoporous silica nanoparticles, method of synthesis and use as a nanovector

A method for self-assembling a mesoporous silica nanoparticle. The method comprises the step of condensing a silica precursor, a surfactant and a condensation agent in a solvent. Then, the addition of an organotriethoxysilane is performed. Finally, there is the step of removing the surfactant. The method is remarkable in that the portion of the organotriethoxysilane to the silica precursor is comprised between 5% and 15%. Additionally, a self-assembled mesoporous silica nanoparticle comprising at least one silica precursor and an organotriethoxysilane.

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Gorcy, France

Jean-Sebastien Thomann