Imec Vzw

Universiteit Gent

Universiteit Antwerpen

Jean-Pierre Timmermans

Wouter Van Putte

Jan Vanfleteren

Thomas Reichert

The invention provides in an electron microscopy grid, comprising: a perforated substrate; a support film on the perforated substrate; a mixture of different linker molecules according to Structure (I), wherein AG is an anchoring group, for anchoring the linker molecule to the solid support; BU is a binding unit, for binding to the analyte; L1 is a first linear linker section; Lis a second linear linker section; α is the angle between the linear linker section Land the linear linker section L; AS is an angled linker section, connecting the linear linker section Land the linear linker section L. The invention further provides in method of structural determination of analytes, using such EM-grids.

Electron microscopy grids and high-resolution structural determination methods

An electron microscopy grid, includes: (i) a perforated substrate, (ii) a support film on the perforated substrate, the support film having a thickness of 60 Å or less, and (iii) linkers attached on top of the support film. The linkers has at least one affinity group for immobilizing an analyte; wherein the linkers form a non-random pattern on the support film.

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Mol, Belgium

Jean-Pierre Timmermans