Centre National De La Recherche Scientifique

Institut National De La Sante Et De La Recherche Medicale

Sorbonne Université

Université Paris—sud

Odyssee Environnement

Université Abderrahmane Mira

Jean Olivier Gamby

Bernard Tribollet

Zineb Belarbi

Boualem Saidani

Bruno Sotta

Laid Makhloufi

Sebastien Meance

Jean-Michel Siaugue

Antoine Pallandre

Vincent Dupuis

Lila Chaal

Anne-Marie Haghiri-Gosnet

Marie-Charlotte Horny

Daniel Rose

Fabrice Guenole Chaussec

Mathieu Lazerges

A process for pre-concentration and detection of at least one single-stranded nucleic acid target molecule, the process comprising the steps of generating a flow of liquid comprising at least one magnetic nanoparticle in a micro-channel and a plurality of single-stranded nucleic acid probe molecules attached to the nanoparticle, generating an alternating magnetic field in the part of the micro-channel using an electromagnet, the magnetic field having an intensity and frequency that are suitable for causing magnetic hyperthermia of the nanoparticles so as to cause denaturing of the duplex formed by the single-stranded nucleic acid target molecule and the single-stranded nucleic acid probe molecule, and detecting the single-stranded target molecule dispersed in the liquid.

Process for detecting nucleic acid molecules by magnetic hyperthermia and assembly enabling such detection

The present invention relates to a microfluidic analysis device () including: a substrate () wherein a separation channel () is arranged, in which an electrolyte flows, a portion of the separation channel () being covered with a polarizable surface (); two longitudinal field electrodes () arranged on either side of the separation channel (); at least one control electrode () positioned in the separation channel (), the control electrode () being suitable for polarizing the polarizable surface () so as to control the speed of the electro-osmotic flow in the separation channel (); the microfluidic analysis device () being characterised in that the polarizable surface () includes an insulating sub-layer () made of amorphous silicon carbide (SiC) and an upper polarizable layer () in direct contact with the electrolyte, the control electrodes () being positioned between the insulating sub-layer () and the upper polarizable layer ().

Microfluidic analysis device and method

This invention concerns the process for the manufacture of an 'anti-lime scaling' product, that is, a product designed to inhibit the formation of lime scale in elements such as pipes or hoses that could be subject to deterioration over time due to lime scale deposits. Specifically, the process of the invention is for an ecological anti-lime scale product. For this, the process according to the invention comprises a preparation step () of vegetal matter from the Caryophyllacae family, an infusion step () of this vegetal matter, a filtration step () of this infusion and a preparation step () of the anti-lime scale solution from a filtrate obtained during the filtration step.

Manufacturing process for a vegetal matter-based anti-lime scale product

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Paris, France

Jean Olivier Gamby