Centre National De La Recherche Scientifique

Other

Institut National De La Recherche Agronomique

Université Libre De Bruxelles

Universitéde Liége

Institut National De La Recherche Agronomique (inra)

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Carbios

Institut National Des Sciences Appliquees De Toulouse

Institut National Des Sciences Appliquees

Institut National De Recherche Pour L'agriculture, A'alimentation Et L'environnement

Institut Francais Du Pe'trole

Ifp Energies Nouvelles

Institut National De La Recherche Scientifique

Centre National De La Recherche Scientifique-cnrs

Jean-Marc Nicaud

Athanasios Beopoulos

Alain Marty

Jonathan Verbeke

Florence Bordes

Georges Pignede

Remy Marchal

Marie Guicherd

Claude Gaillardin

Sophie Lajus

France Thevenieau

Marion Trassaert

Frederic Carly

Marie Vandermies

Patrick Fickers

Stephane Thomas

Nigel Mackman

Ian B Holland

Stéphane Thomas

Rémy Marchal

Thierry Chardot

Jean-Marie Beckerich

Cécile Neuveglise

Franck Fudalej

Thierry Dulermo

Vinciane Borsenberger

Simon Dusseaux

Cecile Neuveglise

Marie-Therese Le Dall

Frédéric Carly

Marie-Thérèse Le Dall

The invention is related to an inducible promoter for improved and regulated gene expression, useful in synthetic biology and metabolic engineering. In particular, the present invention relates to a nucleotide sequence comprising the regulatory regions of an erythritol- and erythrulose-inducible promoter in yeast and uses thereof in an expression system thus allowing an improved and regulated gene expression and production of gene product.

Inducible promoter for gene expression and synthetic biology

The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof.

Recombinant yeast cells producing polylactic acid and uses thereof

The present invention relates to a method for obtaining a mutant strain of oleaginous yeast which is useful as a template strain of yeast for obtaining other mutant strains of oleaginous yeast which are capable of producing an unusual fatty acid. The present invention also relates to the mutant strains of yeast obtained by said method.

Mutant yeasts capable of producing an unusual fatty acid

This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of astrain including the steps of: (a) cultivating astrain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming saidstrain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors. This invention also includes a method for producing a polypeptide of interest using this method as well as a method for obtaining a modifiedstrain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain.

Method for the targeted integration of multiple copies of a gene of interest in a yarrowia strain

The subject of the present invention is a novel mutant yeast strain, particularly astrain, capable of accumulate a large quantity of lipids. The said strain does not express the GUT2 gene. According to one variant, the invention relates to mutant yeast strains, particularlystrains, which do not express the GUT2 gene and which furthermore do not carry out the &#x3b2;-oxidation of lipids. Preferably, the mutant strains which do not express the GUT2 gene and which furthermore do not carry out &#x3b2;-oxidation of lipids do not express the POX 2 through POX 5 genes, most preferably the POX 1 through 6 genes. The subject of the invention is also a method for producing the strains according to the invention and the use of the said strains in the production of lipids.

Mutant yeast strains capable of accumulating a large quantity of lipids

The invention concerns the integration of a gene of interest into the genome of a Yarrowia strain devoid of zeta sequences, by transforming said strain using a vector bearing zeta sequences.

Method for non-homologous transformation of Yarrowia lipolytica

Process for the production of a polypeptide

Growing community of inventors

Trappes, France

Jean-Marc Nicaud