Ecole Polytechnique

Jean-Louis Martin

Emmanuel Jean-Marc Beaurepaire

Delphine Debarre

Nicolas Olivier

Emmanuel Beaurepaire

Marie-Claire Schanne-Klein

Thibault Vieille

Israel Veilleux

Delphine Malvina Daniele Marie Debarre

A method and system for calibrating an optical device having a spatial optical modulator () and including: an optical lens () of a large numerical aperture, capable of receiving an incident beam and/or collecting an optical beam reflected by a sample; a camera () disposed in a plane optically conjugate with the focal plane of the lens (); and a spatial optical modulator () disposed on the optical beam upstream of the camera (). The method includes the following steps consisting in: acquiring three-dimensional images of the PSF in terms of intensity in a plane conjugate with the focal plane of the lens (), reconstructing the profile in terms of amplitude and/or phase of the beam using a phase diversity algorithm, and determining the respective influence functions of a plurality of elements P, . . . , Pof the spatial optical modulator () on the profile in terms of the amplitude A and/or phase &#x3c6; of the beam.

Method and system for calibrating a spatial optical modulator in an optical microscope

A method for acquiring signals in laser scanning microscopy, includes the steps of: moving a focused optical excitation beam relative to an object to be measured so that the focus point of the beam follows a predetermined path in the space of the object; and acquiring optical measurement signals along the path according to at least one acquisition parameter; characterized in that the path of the excitation beam is determined so as to substantially minimize the variations of the optical properties of at least one portion of the environments crossed by the excitation beam between consecutive acquisitions, and in that at least one acquisition parameter among the acquisition parameters is modulated during the movement of the excitation beam. A device for implementing the method is also described.

Method and device for acquiring signals in laser scanning microscopy

A method for the dimensional characterization of a structured material, in which method: an excitation laser beam suitable for coherent nonlinear microscopy is generated, this excitation laser beam being focused in a focal volume within the structured material; signals emitted by the structured material are detected; a plurality of emission patterns, each corresponding to one particular shape of the focal volume, is produced, the particular shapes being obtained for various non-Gaussian spatial profiles of the excitation laser beam wavefront; and on the basis of the emission patterns thus produced, dimensional characteristics of the structured material are deduced therefrom.

Coherent nonlinear microscopy system and method with variation of the focal volume in order to probe the nanostructure of organized materials

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Bures sur Yvette, France

Jean-Louis Martin