Institut Merieux

Khorionyx

Jean L Tayot

Michel A Tardy

Paule A Gattel

Jean L Veron

An implantable preparation comprises a material which can be obtained from globin that has been modified, especially chemically, to be, at least partially, soluble at physiological pH. The material is biocompatible, and biodegradable in the organism. The material may be soluble at physiological pH, or insoluble at that pH. The preparation may be in the form of a solution, suspension, paste, gel, film, sponge, powder or granules, or a solid implant. The preparation can be used for the healing, protection or filling of external skin wounds, the filling of wrinkles and skin flaws, the filling of tissue, as a device for fixing prostheses or biomaterials, or as a device for preventing adhesion.

Implantable preparations

The process comprises subjecting the hemolyzed blood to a clarification step at a pH of between 4 and 6 in the presence of less than 15% of alcohol, then, after concentration, effecting a chromatography by exchange of anions so as to separate the hemoglobin, the albumin being thereafter eluted. The hemoglobin and the globulins may then be separated out by precipitation with 25% alcohol at a pH of 7. The proteins obtained are in the native state.

Process for preparing the principal proteins of hemolyzed blood in the

An 80% alcoholic solution of haemoglobin having a pH close to 3 is adsorbed on active charcoal at less than 20.degree. C. while continuously stirring and the globin solution obtained is separated out by centrifuging or filtering. The globin obtained is depigmented.

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La Tour de Salvagny, France

Jean L Tayot