Aventis Pharma S.a.

Rhone-poulenc Rorer S.a.

Jean-Jacques Robert

Jacques Mallet

Michel Perricaudet

Axel Kahn

Marc Peschanski

Gildas Le Gal La Salle

Patrice Yeh

Joel Crouzet

Emmanuelle Vigne

Frederic Revah

The invention concerns compositions and methods for preparing recombinant adenoviruses. The resulting adenoviruses can be used for transferring and/or expressing genes in cells, in vitro, ex vivo or in vivo, or also in functional genomics. More particularly, the invention concerns in particular efficient methods for producing adenovirus banks and the use of said banks in functional genomics. The invention also concerns plasmids used for constructing said adenoviruses.

Recombinant adenoviruses preparation and adenovirus banks

The invention concerns a recombinant DNA vector characterized in that it is capable of directing the expression an/or transcription of a selected nucleotide sequence in the cells of the central nervous system and in that it comprises (i) at least part of the genome of an adenovirus, including the regions required for that adenovirus to penetrate into the cells normally infectable by that adenovirus and (ii) being inserted into said part of genome of an adenovirus under the control of a promoter, either present or also inserted into said genome part and operative in said cells. This recombinant vector finds particular use in the treatment of diseases of the central nervous system, also in gene therapy.

Adenovirus vectors for the transfer of foreign genes into cells of the central nervous system particularly in brain

Assays for promoter operability in central nervous system cells

The invention concerns a method for reducing recombination phenomena among nucleic acids. It also concerns the use of said method for producing defective viruses not contaminated by replication particles. The invention further concerns novel viral constructs.

Adenovirus vectors and method for reducing homologous recombination phenomena

Pharmaceutical compositions and their use, namely for the treatment of

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Sceaux, France

Jean-Jacques Robert